内质网应激相关因子PERK和ATF6在结肠癌中的表达及意义

发布时间：2018-04-17 10:57

本文选题：蛋白激酶R样内质网调节激酶 + 活化转录因子　；参考：《安徽医科大学学报》2017年09期

【摘要】：目的探讨内质网应激相关因子蛋白激酶R样内质网调节激酶(PERK)和活化转录因子6(ATF6)在结直肠癌组织中的表达情况,分析PERK和ATF6在结肠癌发生发展中的作用。方法选择手术切除结肠癌组织及距离病变组织5 cm以上正常组织,采用实时荧光定量PCR技术(RTPCR)检测PERK和ATF6的mRNA表达情况,免疫组织化学法(IHC)、Western blot法检测PERK和ATF6蛋白的表达情况,并结合临床病理特征,分析其与结肠癌发生、发展之间的关系。结果肿瘤组织ATF6和PERK mRNA表达均较正常组织下调(P0.05)。IHC和Western blot结果显示PERK和ATF6在结肠癌中的表达均显著低于正常组织(P0.05)。PERK和ATF6主要定位于上皮细胞中。结论PERK和ATF6在结肠癌中的表达水平下降说明缺乏适当的内质网应激反应可能在肿瘤的发生机制中起作用。
[Abstract]:Objective to investigate the expression of ER stress-related factor protein kinase R-like endoplasmic reticulum regulated kinase (ERK) and activated transcription factor 6 (ATF6) in colorectal carcinoma and to analyze the role of PERK and ATF6 in the carcinogenesis and development of colon cancer.Methods the tissues of colon cancer and normal tissues more than 5 cm away from the lesion were resected. The expression of PERK and ATF6 was detected by real-time fluorescence quantitative PCR and the expression of PERK and ATF6 protein was detected by immunohistochemistry.Combined with clinicopathological features, the relationship between colonic cancer and the occurrence and development of colon cancer was analyzed.Results the expression of ATF6 and PERK mRNA in tumor tissues was significantly lower than that in normal tissues. The results showed that the expression of PERK and ATF6 in colon cancer was significantly lower than that in normal tissues. P0.05, PERK and ATF6 were mainly localized in epithelial cells.Conclusion the decreased expression of PERK and ATF6 in colon cancer suggests that the lack of proper endoplasmic reticulum stress may play a role in the pathogenesis of cancer.
【作者单位】：河南科技大学临床医学院;河南科技大学第一附属医院消化内科;河南科技大学第一附属医院肝胆外科;河南科技大学第一附属医院检验科;首都医科大学附属北京康复医院消化内科;
【基金】：国家自然科学基金(编号:81370487)
【分类号】：R735.35

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