EZH2表达与结直肠癌肿瘤细胞增殖和血管生成及预后的关系

发布时间：2018-04-18 12:41

本文选题：结直肠癌 + EZH　；参考：《中山大学学报(医学科学版)》2017年06期

【摘要】：【目的】探讨果蝇zeste基因增强子同源物2(EZH2)表达与结直肠癌肿瘤细胞增殖、血管生成及预后的关系。【方法】采用免疫组织化学Max Vision两步法检测110例手术切除的原发性结直肠腺癌组织中EZH2、增殖细胞核抗原(Ki-67)和CD34标记血管内皮细胞计数肿瘤微血管密度(i MVD)的表达情况,并分析其与结直肠癌临床病理特征的关系。用Pearson相关系数分析EZH2、Ki-67及i MVD表达的相关性。用Kaplan-Meier法分析生存曲线,多变量Cox比例风险回归模型筛选影响患者生存的独立预后因素。【结果】结直肠癌组织的EZH2、Ki-67及i MVD阳性表达率分别为40.91%(45/110)、57.27%(63/110)和46.36%(51/110)。结直肠癌远处转移患者的EZH2蛋白阳性表达率高于无远处转移患者(P=0.024)。Ki-67与原发灶大小、肠壁浸润深度、AJCC分期、分化程度密切相关(P=0.033~0.015)。i MVD与肿瘤的肠壁浸润深度、AJCC分期、分化程度密切相关(P=0.016~0.034)。Spearman相关分析显示EZH2与Ki-67呈正相关(r=0.195,P=0.041),而与i MVD的表达无关;Ki-67与i MVD呈正相关(r=0.213,P=0.025)。多因素分析结果表明,EZH2蛋白的表达是结直肠癌患者预后差的独立影响因素(HR 1.965,95%CI 1.019~3.789,P=0.044)。【结论】结直肠癌组织中EZH2过表达与肿瘤细胞的增殖活性有关,在一定程度上EZH2具有判断结直肠癌切除术后转移和预后的价值。
[Abstract]:[objective] to investigate the expression of zeste gene enhancer 2EZH2) and the proliferation of colorectal cancer cells in Drosophila melanogaster.Relationship between angiogenesis and prognosis. [methods] Immunohistochemical Max Vision two-step method was used to detect EZH2, proliferating cell nuclear antigen Ki-67 (PCNA Ki-67) and CD34 labeled endothelial cell count in 110 cases of primary colorectal adenocarcinoma.Expression of microvessel density I MVD in tumor,The relationship between it and clinicopathological features of colorectal cancer was analyzed.The correlation between the expression of Ki-67 and I MVD was analyzed by Pearson correlation coefficient.The survival curve was analyzed by Kaplan-Meier method and independent prognostic factors were screened by multivariate Cox proportional risk regression model. [results] the positive expression rates of EZH2Ki-67 and I MVD in colorectal cancer tissues were 40.91% 45 / 110%, 57.27% 63 / 110) and 46.36% 51 / 11010, respectively.The positive expression rate of EZH2 protein in patients with distant metastasis of colorectal cancer was higher than that in patients without distant metastasis. Ki-67 was correlated with the size of primary tumor, the depth of invasion of intestinal wall and the stage of AJCC. The degree of differentiation was closely related to the depth of invasion of the tumor and the depth of invasion of the tumor.The degree of differentiation was closely related to the degree of differentiation. Spearman correlation analysis showed that there was a positive correlation between EZH2 and Ki-67, while there was no positive correlation between Ki-67 and I MVD expression.The results of multivariate analysis showed that the expression of EZH2 protein was an independent factor influencing the prognosis of colorectal cancer patients. [conclusion] the overexpression of EZH2 in colorectal cancer is related to the proliferation activity of tumor cells.To some extent, EZH2 has the value of judging the metastasis and prognosis of colorectal cancer after resection.
【作者单位】：中山大学附属第五医院胃肠外科;
【基金】：广东省珠海市科技计划项目(20171009E030027)
【分类号】：R735.34

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