OTUB1在90例卵巢黏液性肿瘤中的表达分析

发布时间：2018-04-20 12:34

本文选题：泛素醛结合物 + 卵巢　；参考：《中国癌症杂志》2017年06期

【摘要】：背景与目的:卵巢黏液性肿瘤是卵巢上皮性肿瘤的主要亚型之一,其恶变机制目前尚未明确。泛素化是人体主要的翻译后修饰机制,并与肿瘤密切相关。去泛素化酶可通过逆转泛素化过程从而影响肿瘤的发生、发展。该研究旨在分析编码人体最主要的去泛素化酶的癌基因泛素醛结合物1(OTU deubiquitinase,ubiquitin aldehyde binding 1,OTUB1)在卵巢黏液性肿瘤中的蛋白表达及临床应用。方法:收集2010—2015年复旦大学附属妇产科医院病理科诊断的卵巢良性黏液性囊腺瘤、卵巢交界性黏液性肿瘤及卵巢原发性黏液性癌病例共90例,收集临床病理资料,完成OTUB1的免疫组织化学法表达实验并作统计分析。结果:90例卵巢黏液性肿瘤中,卵巢良性黏液性囊腺瘤有14例,卵巢交界性黏液性肿瘤有17例,卵巢原发性黏液性癌有59例。OTUB1在卵巢原发性黏液性癌的蛋白表达率及阳性程度显著高于卵巢良性黏液性囊腺瘤(P0.01);OTUB1在卵巢交界性黏液性肿瘤中上皮内癌的阳性率显著高于肠型交界性黏液性肿瘤(P0.01);随着FIGO分期增高,OTUB1的阳性率及阳性程度增加(P0.05);OTUB1在输卵管有累及的患者中表达率及阳性程度均高于未累及者(P0.05);OTUB1在子宫和大网膜累及的患者中阳性率及阳性程度均高于未累及者(P0.05);OTUB1在有淋巴结转移的患者中表达率及阳性程度均高于无转移者(P0.05)。结论:OTUB1在卵巢原发性黏液性癌中的蛋白表达水平明显高于卵巢良性黏液性囊腺瘤,并且和FIGO分期及肿瘤侵袭转移性高度相关。在临床病理中可将OTUB1作为卵巢黏液性肿瘤恶变与否的评价指标及肿瘤进展的辅助判别指标。
[Abstract]:Background & AIM: ovarian mucinous tumor is one of the main subtypes of epithelial ovarian tumor. Ubiquitin is the main posttranslational modification mechanism in human body and is closely related to tumor. De-ubiquitin enzyme can influence the development of tumor by reversing the process of ubiquitin. The aim of this study was to analyze the protein expression and clinical application of 1(OTU ubiquitin aldehyde binding 1 OTUB1, a major oncogene ubiquitin aldehyde conjugate encoding the most important deubiquiase in human ovarian mucinous tumors. Methods: a total of 90 cases of benign ovarian mucinous cystadenoma, borderline ovarian mucinous tumor and primary ovarian mucinous carcinoma were collected from the Department of Pathology, Department of Pathology, affiliated Obstetrics and Gynecology Hospital of Fudan University from 2010 to 2015. The clinicopathological data were collected. The immunohistochemical expression of OTUB1 was carried out and statistical analysis was made. Results among 90 cases of ovarian mucinous tumors, 14 cases were benign ovarian mucinous cystadenoma and 17 cases were borderline ovarian mucinous tumors. The protein expression and positive degree of OTUB1 in ovarian primary mucinous carcinoma were significantly higher than that of benign ovarian mucinous cystadenoma P0.01OTUB1 in ovarian borderline mucinous tumor. The positive rate of OTUB1 in ovarian borderline mucinous carcinoma was significantly higher than that in ovarian benign mucinous cystadenoma. The positive rate and degree of OTUB1 in patients with fallopian tube involvement were higher than those in patients with tubal involvement, and the positive rate and degree of OTUB1 in uterus and omentum were higher than those in patients without tubal involvement, and the positive rate and degree of OTUB1 in uterus and omentum were higher than those in patients with intestinal borderline mucinous neoplasms (P 0.01), and the positive rate and degree of OTUB1 in uterus and greater omentum were higher than those in patients with fallopian tube involvement. The positive rate and degree of OTUB1 in patients with lymph node metastasis were higher than those without metastasis. Conclusion the protein expression level of OTUB1 in primary mucinous ovarian carcinoma is significantly higher than that in benign ovarian mucinous cystadenoma, and is highly correlated with FIGO stage and tumor invasion and metastasis. In clinicopathology, OTUB1 can be used as an index to evaluate the malignancy of ovarian mucinous tumor and as an auxiliary discriminant index of tumor progression.
【作者单位】：复旦大学附属妇产科医院病理科;
【基金】：国家自然科学基金会青年项目(81602269)
【分类号】：R737.31

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