纳米孔道单分子检测结直肠癌MicroRNAs

发布时间：2018-04-21 22:19

本文选题：microRNA + α-溶血素　；参考：《化学学报》2017年11期

【摘要】：MicroRNA(miRNA)可用于癌症的早期诊断、预后判断,其分析检测具有重要临床意义.结直肠癌的发生、发展与miRNA 21、miRNA 92等的异常表达明显相关.本研究设计了以poly(d T)n为引导链的DNA探针(probe)并尝试使用α-溶血素(α-HL)纳米孔道单分子检测方法检测结直肠癌miRNAs.miRNA·probe复合物分子穿过α-HL纳米孔道限域空间时,由于probe链长、序列不同导致probe-α-HL相互作用不同,miRNA 92·probe 92、miRNA 21·probe 21、miRNA 16·probe 16输出为形态、阻断时间不同的多台阶特征信号,实现了三种miRNAs的有效区分.实验证明,此方法可以用于检测血清实际样品.因此,未来有望使用α-HL构建miRNA超灵敏单分子生物传感器.
[Abstract]:MicroRNA (miRNA) can be used for early diagnosis and prognosis of cancer, and its analysis and detection have important clinical significance. The occurrence and development of colorectal cancer are closely related to the abnormal expression of miRNA 21, miRNA 92. This study designed a DNA probe (probe) using poly (D T) n as the guiding chain and attempted to use alpha hemolysin (alpha -HL) nanopore single molecule detection. When the miRNAs.miRNA / probe complex molecules of colorectal cancer pass through the limit space of the -HL channel of the alpha -HL, the probe chain is long and the sequence is different, which leads to the different interaction of probe- alpha -HL, miRNA 92. Probe 92, miRNA 21. Probe 21, miRNA 16. Probe 16 is the form, and the interruption time is different step characteristic signal, and three kinds of signals are realized. The effective differentiation of NAs shows that this method can be used to detect the actual serum samples. Therefore, it is expected that the miRNA ultra sensitive single molecule biosensor can be constructed by using alpha -HL in the future.

【作者单位】：华东理工大学化学与分子工程学院结构可控先进功能材料及其制备教育部重点实验室;深圳大学附属南山医院中心实验室;
【基金】：国家重点研发计划(No.2017YFC0906500) 国家自然科学基金(No.21505043) 上海市教育委员会科研创新项目(No.2017-01-07-00-02-E00023) 中央高校基本科研业务费(Nos.222201714012,222201718001,222201717003)资助~~
【分类号】：R735.3;TP212

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