SIRT-1在不同宫颈组织中的表达及其临床意义

发布时间：2018-04-24 22:17

本文选题：Ⅲ型去乙酰化酶SIRTUIN + 免疫组化　；参考：《实用妇产科杂志》2017年09期

【摘要】：目的:探讨Ⅲ型去乙酰化酶SIRTUIN1(SIRT-1)蛋白在正常宫颈组织及不同级别宫颈病变组织中的表达及其临床意义。方法:应用Western-blot方法及免疫组化(SP)法检测正常宫颈组织(24例)、低级别鳞状上皮内病变组织(LSIL)26例、高级别鳞状上皮内病变组织(HSIL)35例及宫颈癌组织55例中SIRT-1蛋白的表达情况;并分析SIRT-1蛋白的表达与宫颈癌组织各临床病理参数间的关系。结果:(1)SIRT-1蛋白的高表达率在正常宫颈组织为0、LSIL为15.38%、HSIL为37.14%及宫颈癌组织为40.00%,差异有统计学意义(χ~2=16.82,P0.05);(2)宫颈癌组织中SIRT-1蛋白的高表达率在不同组织学分化程度(G1~G3)(χ~2=6.87,P0.05)、有无淋巴结转移(χ~2=7.73,P0.05)、不同临床分期(χ~2=7.39,P0.05)间差异均有统计学意义。结论:SIRT-1随宫颈病变的加重表达逐渐增强,与宫颈癌组织学分化程度、有无淋巴结转移、临床分期有关。可能在宫颈癌的发生和发展过程中起促进作用。
[Abstract]:Objective: to investigate the expression and clinical significance of SIRTUIN1 SIRT-1 protein in normal cervical tissues and cervical lesions of different grades. Methods: the expression of SIRT-1 protein in 24 cases of normal cervical tissues, 26 cases of low grade squamous intraepithelial lesions, 35 cases of high grade squamous intraepithelial lesions and 55 cases of cervical carcinoma were detected by Western-blot and immunohistochemical staining. The relationship between the expression of SIRT-1 protein and the clinicopathological parameters of cervical carcinoma was analyzed. Results the high expression rate of SIRT-1 protein in normal cervix was 15.38% in normal cervix and 40.005% in cervical carcinoma. There was significant difference in high expression rate of SIRT-1 protein in cervical carcinoma tissues with different histological differentiation degree (蠂 ~ 2 ~ 2 ~ (6.87) P ~ (0.05) P ~ (0.05)). There were statistically significant differences in different clinical stages (蠂 ~ 2 ~ (2) ~ (7.39) ~ P ~ (0.05)) among patients with metastasis (蠂 ~ (2) = 7.73) or P ~ (0.05). Conclusion the increased expression of 1% SIRT-1 is related to histological differentiation, lymph node metastasis and clinical stage of cervical carcinoma. May play a promoting role in the occurrence and development of cervical cancer.
【作者单位】：青岛市妇女儿童医院;青岛大学附属医院;
【基金】：山东省自然科学基金(编号:ZR2015HM035)
【分类号】：R737.33

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