纳米银对急性乏氧U251细胞的影响及对其放疗增敏与自噬的研究

发布时间：2018-04-25 22:11

本文选题：纳米银 + 乏氧　；参考：《南京航空航天大学》2017年硕士论文

【摘要】：纳米银作为一种抗肿瘤剂,可广泛应用于肿瘤治疗。大多数肿瘤内都存在乏氧细胞,其抵抗射线,使治疗效果差。同样,人脑胶质瘤内缺氧不可避免。之前有体外细胞实验证实,纳米银能增强人神经胶质瘤细胞对放射线的敏感性。然而,纳米银对乏氧胶质瘤细胞的作用仍不清楚。本文讨论了纳米银对乏氧胶质瘤U251细胞的影响。采用乏氧装置体外培养急性乏氧细胞,用CCK-8实验检测纳米银对细胞的毒性;随后,将胶质瘤细胞分为对照组、辐射组、单纯纳米银组和联合治疗组。四组分别对细胞凋亡、周期、克隆形成、线粒体膜电位、自噬及活性氧等进行检测,分析纳米银与乏氧细胞共孵育后,细胞对放射线的敏感性及纳米银、辐照对乏氧细胞自噬的影响。结果表明:1.用纳米银处理的乏氧胶质瘤细胞存在毒性剂量效应,出于对细胞存活率的考虑,选取15μg/mL的纳米银进行之后研究。2.纳米银对急性乏氧后的U251细胞有放疗增敏效果,并找到了治疗急性乏氧U251细胞的最佳射线强度。3.使用自噬抑制剂3-MA验证,纳米银/辐射都能诱导急性乏氧U251细胞自噬,且其引起的自噬对急性乏氧细胞起抑制细胞存活作用。4.同样条件下,纳米银还引起急性乏氧细胞产生活性氧,造成线粒体膜电位损伤及引发细胞凋亡。本文结果显示,纳米银可有效地增加急性乏氧脑胶质瘤对射线的敏感性,对纳米银引起乏氧细胞自噬表达升高及其机制研究具有理论意义,可为纳米银在肿瘤放疗领域的应用提供依据。
[Abstract]:As an anti-tumor agent, nano-silver can be widely used in tumor therapy. Hypoxia cells are present in most tumors, which resist radiation and make the treatment ineffective. Similarly, hypoxia in human gliomas is inevitable. Previous in vitro cell experiments have confirmed that silver nanoparticles can enhance the radiosensitivity of human glioma cells to radiation. However, the effect of silver nanoparticles on hypoxic glioma cells remains unclear. The effect of nano silver on hypoxia glioma U251 cells was discussed in this paper. Acute hypoxia cells were cultured in vitro with hypoxia apparatus. The toxicity of silver nanoparticles to cells was detected by CCK-8 assay. Then glioma cells were divided into control group radiation group simple nano-silver group and combined treatment group. Apoptosis, cell cycle, clone formation, mitochondrial membrane potential, autophagy and reactive oxygen species were detected in the four groups. Effects of irradiation on autophagy of hypoxic cells. The result shows that 1: 1. The hypoxic glioma cells treated with nano-silver had toxic dose effect. For the sake of cell survival rate, 15 渭 g/mL nano-silver was selected to carry out the study. Silver nanoparticles can sensitize U251 cells after acute hypoxia and find the best radiation intensity. 3. Using autophagy inhibitor 3-MA, nano-silver / radiation could induce acute hypoxia U251 cell autophagy, and its induced autophagy could inhibit the survival of U251 cells. Under the same conditions, silver nanoparticles also induced reactive oxygen species (Ros) production in acute hypoxia cells, resulting in mitochondrial membrane potential damage and apoptosis. The results show that nano-silver can effectively increase the radiosensitivity of acute hypoxic gliomas, and has theoretical significance in the study of the mechanism of the increase of autophagy expression induced by nano-silver. It can provide the basis for the application of nano silver in tumor radiotherapy.
【学位授予单位】：南京航空航天大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R730.55

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