P-选择素、TPO、IL-6及血小板与非小细胞肺癌的相关性研究

发布时间：2018-04-28 08:02

  本文选题：非小细胞肺癌 + 白介素6　； 参考：《贵州医科大学》2017年硕士论文

【摘要】：目的:分析P-选择素、TPO、IL-6及血小板与非小细胞肺癌(NSCLC)的相关性。方法:选取2015年11月至2016年11月期间在贵州医科大学附属医院和贵州医科大学附属肿瘤医院被确诊为NSCLC患者50例为NSCLC组,并以同期体检健康者50例作为对照组。应用酶联免疫吸附法(ELISA)和全自动血细胞分析仪分别检测患者术前和术后3-4周以及健康体检者的血清P-选择素、IL-6、TPO浓度以及PLT数量;免疫组织化学(IHC)用于检测患者术后癌组织及癌旁组织中P-选择素、IL-6、TPO的表达情况。然后应用SPSS19.0软件对以上指标的检测数据进行统计学分析。结果:NSCLC组的术前外周血血清P-选择素、IL-6、TPO、PLT水平均高于健康对照组(P0.05);术后外周血血清IL-6、P-选择素和TPO的浓度明显低于术前(P0.05);癌组织中免疫组化IL-6阳性表达者血清IL-6水平明显高于阴性患者(P0.05);免疫组化阳性表达者血清P-选择素水平高于阴性患者,但差异没有统计学意义(P0.05)。在NSCLC患者中,外周血IL-6、TPO及PLT随着病情的加重(分期和淋巴结转移)有升高的趋势(P0.05),且与吸烟指数有关(P0.05),与病理分型无关(P0.05);而P-选择素的水平只与TNM分期和病理分型有关,且Ⅲ、Ⅳ期的患者水平明显高于Ⅰ、Ⅱ期患者,腺癌患者高于鳞癌而与其他因素无明显相关性。肺癌组织中的IL-6表达明显高于癌旁组织(P0.05),并且与吸烟指数和病情严重程度有相关性(P0.05),但与肿瘤大小和组织分型无关(P0.05);肺癌组织中的P-选择素表达明显高于癌旁组织(P0.05),只与病理分期和组织类型有关(P0.05);TPO在肺癌组织中未见明显表达。非小细胞肺癌组TPO、PLT、IL-6血清水平两两之间存在不同程度的相关性。其中,TPO与PLT的相关系数最高。结论:NSCLC患者体内IL-6、TPO、P-选择素、PLT升高对病情评估有一定的临床意义;NSCLC患者IL-6的升高与IL-6在肿瘤组织中的高表达有关;IL-6与TPO可能参与了NSCLC患者中血小板数量的改变。NSCLC患者P-选择素的表达水平可以协助判断的病理分型、远处转移能力及预后。
[Abstract]:Aim: to analyze the correlation between P-selectin TPO 6-6 and platelets and NSCLC in patients with non-small cell lung cancer (NSCLC). Methods: from November 2015 to November 2016, 50 patients with NSCLC in affiliated Hospital of Guizhou Medical University and affiliated Cancer Hospital of Guizhou Medical University were selected as NSCLC group, and 50 healthy persons as control group. The serum levels of P- selectin IL-6 TPO and the number of PLT were measured by Elisa and automatic blood cell analyzer before and 3 to 4 weeks after operation and 3 to 4 weeks after operation, respectively. Immunohistochemistry was used to detect the expression of P- selectin IL-6 TPO in cancer tissues and paracancerous tissues after operation. Then the SPSS19.0 software is used to carry on the statistical analysis to the above index detection data. Results the serum levels of P- selectin, IL-6, TPO-PLT were higher than those of healthy control group before operation, and the levels of serum IL-6, P- selectin and TPO in postoperative peripheral blood were significantly lower than those before operation, and the levels of serum IL-6 in patients with positive expression of immunohistochemical IL-6 in cancer tissues were significantly lower than those in the control group. The level of serum P- selectin in the patients with positive immunohistochemical expression was higher than that in the patients with negative staining. But the difference was not statistically significant (P 0.05). In patients with NSCLC, the levels of IL-6TPO and PLT in peripheral blood increased with the severity of the disease (stage and lymph node metastasis), and were related to smoking index (P0.05), not to pathological type (P0.05), but the level of P- selectin was only related to TNM stage and pathological type, and 鈪，

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