SIRT1在前列腺癌发生发展中的作用及机制研究

发布时间：2018-04-28 10:56

本文选题：SIRT1 + 前列腺癌　；参考：《兰州大学》2017年硕士论文

【摘要】：目的:了解沉默信息调节因子1(silent information regulator 1,SIRT1)在前列腺癌组织、前列腺增生组织、正常前列腺组织中的表达情况,研究SIRT1在前列腺癌细胞生物学中的作用,并初步探讨SIRT1在前列腺癌中的作用机制。为前列腺癌诊治、复发检测、预后等提供新的靶点和策略。方法:本研究用免疫组织化学方法检测SIRT1在前列腺癌、良性前列腺增生、前列腺正常组织中的表达情况。检索肿瘤数据库ONCOMINE,研究SIRT1在前列腺癌中的表达情况。用SIRT1特异性抑制剂Nicotinamide、SIRT1 siRNA使前列腺癌中SIRT1表达下调后,研究对前列腺癌细胞增殖能力的影响。用SIRT1siRNA转染LNcaP细胞后,用流式细胞仪检测SIRT1表达下调后对前列腺癌细胞周期和细胞凋亡的影响。结果:与正常前列腺组织、前列腺增生组织相比,SIRT1在前列腺癌组织中高表达。肿瘤数据库ONCOMINE中检索发现SIRT1在前列腺癌中的表达情况不一致。SIRT1特异性抑制剂Nicotinamide、SIRT1 siRNA抑制SIRT1表达后,前列腺癌细胞增殖明显受到抑制。用SIRT1 siRNA转染LNcaP细胞后,流式细胞仪检测发现前列腺癌细胞周期受到抑制和细胞凋亡明显增加。结论:SIRT1通过阻滞前列腺癌细胞周期和增加细胞凋亡促进前列腺癌的发生和进展,SIRT1可为前列腺癌诊治、复发检测和预后等提供新的靶点和方向。
[Abstract]:Objective: to investigate the expression of 1(silent information regulator 1 (SIRT1) in prostate cancer, prostatic hyperplasia and normal prostate, and to study the role of SIRT1 in prostate cancer cell biology. The mechanism of SIRT1 in prostate cancer was also discussed. To provide new targets and strategies for diagnosis and treatment, recurrence detection and prognosis of prostate cancer. Methods: immunohistochemical method was used to detect the expression of SIRT1 in prostate cancer, benign prostatic hyperplasia and normal prostate tissues. To study the expression of SIRT1 in prostate cancer by searching oncomi cancer database. The effect of SIRT1 specific inhibitor Nicotinamideon SIRT1 siRNA on the proliferation of prostate cancer cells was studied after the expression of SIRT1 was down-regulated. After transfection of LNcaP cells with SIRT1siRNA, the effect of down-regulation of SIRT1 expression on cell cycle and apoptosis of prostate cancer cells was detected by flow cytometry. Results: compared with normal prostate tissues and prostatic hyperplasia tissues, sirt1 was highly expressed in prostate cancer tissues. Different expression of SIRT1 in prostate cancer was found in ONCOMINE database. Inhibition of SIRT1 expression by Nicotinamide-SIRT1 siRNA, a specific inhibitor of SIRT1, significantly inhibited the proliferation of prostate cancer cells. After transfection of LNcaP cells with SIRT1 siRNA, the results of flow cytometry showed that the cell cycle of prostate cancer cells was inhibited and apoptosis was significantly increased. Conclusion by blocking the cell cycle and increasing the apoptosis of prostate cancer cell cycle, the cell cycle and progression of prostate cancer can be promoted by 1: SIRT1. SIRT1 may provide a new target and direction for the diagnosis and treatment of prostate cancer, the detection of recurrence and the prognosis of prostate cancer.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.25

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