循环肿瘤细胞检测研究进展:从单纯计数到表型与功能分析

发布时间：2018-04-29 14:34

本文选题：循环肿瘤细胞 + 液体活检　；参考：《中国肿瘤生物治疗杂志》2017年04期

【摘要】：随着靶向治疗、免疫治疗等新技术的发展,肿瘤诊疗已经步入精准医疗的新时代。液体活检作为一种便捷、无创、能够动态反映肿瘤基因谱全貌的方法在肿瘤精准医疗中发挥着越来越重要的作用。血液循环肿瘤细胞(circulating tumor cell,CTC)检测是最早开展的液体活检技术,在肿瘤早期诊断、预后判断、疗效评估等方面获得了广泛应用。初级阶段CTC的检测以单纯计数为主,但人们逐渐发现单纯计数无法真实反映肿瘤的负荷及变化情况。随着单细胞测序等技术的不断发展,人们对CTC的认识也不断深入,逐渐转向以分子表型和功能分析为主的高级阶段。本文结合近年来的临床实践,重点阐述CTC检测从初级阶段发展到高级阶段及其在肿瘤精准医疗中应用的研究进展。
[Abstract]:With the development of targeted therapy, immunotherapy and other new technologies, tumor diagnosis and treatment has entered a new era of precision medicine. Fluid biopsy, as a convenient, noninvasive, dynamic method to reflect the full picture of tumor gene spectrum, plays a more and more important role in accurate treatment of cancer. The detection of circulating tumor cells is the earliest liquid biopsy technique, which has been widely used in the early diagnosis, prognosis judgment and evaluation of curative effect of tumor. In the primary stage, simple count was the main method of detection of CTC, but it was gradually found that simple count could not truly reflect the load and change of tumor. With the development of single cell sequencing and other technologies, people's understanding of CTC is becoming more and more in-depth, gradually turning to the advanced stage of molecular phenotypic and functional analysis. Based on the clinical practice in recent years, this paper focuses on the development of CTC detection from the primary stage to the advanced stage and its application in cancer precise medicine.
【作者单位】：第二军医大学附属长海医院实验诊断科;
【基金】：国家自然科学基金项目(No.81672350,No.81472770,No.81272280) 上海市重点弱势学科支持项目-检验病理学科(No.2015ZB0202)~~
【分类号】：R730.4;;R730.7;;R730.5

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