miR-429过表达对膀胱癌细胞迁移、侵袭能力的影响及机制

发布时间：2018-04-30 01:00

本文选题：膀胱癌 + 微小RNA-　；参考：《山东医药》2017年45期

【摘要】：目的探讨miR-429过表达对膀胱癌细胞迁移、侵袭能力的影响及机制。方法将常规培养好的膀胱癌细胞T24随机分为对照组、miR-429组,对照组转染miR-NC(无关序列的mimics),miR-429组转染插入miR-429的目标片段。用划痕试验检测两组迁移能力,Transwell侵袭试验检测其侵袭能力。采用实时PCR技术检测T24细胞内E盒结合锌指蛋白(ZEB1)、基质金属蛋白酶2(MMP-2)及钙黏附蛋白E(E-cadherin)mRNA表达,用免疫印迹法检测T24细胞内ZEB1、MMP-2及E-cadherin蛋白表达。结果与对照组比较,miR-429组细胞迁移距离短,迁移细胞数少(P均0.05),细胞内ZEB1、MMP-2 mRNA及蛋白表达低,E-cadherin mRNA及蛋白表达高(P均0.05)。结论 miR-429b过表达可能通过靶向抑制膀胱癌细胞内ZEB1、MMP-2表达,增强E-cadherin的表达阻止膀胱癌细胞迁移、侵袭。
[Abstract]:Objective to investigate the effect and mechanism of overexpression of miR-429 on migration and invasion of bladder cancer cells. Methods T24 cells were randomly divided into control group (control group) and control group (control group). The migration ability of two groups was detected by scratch test and the invasion ability was detected by Transwell invasion test. The expression of E-box binding zinc finger protein (ZEB1), matrix metalloproteinase-2 (MMP-2) and calmodulin (E(E-cadherin)mRNA) in T24 cells were detected by real-time PCR technique. The expression of ZEB1MMP-2 and E-cadherin protein in T24 cells was detected by Western blot. Results compared with the control group, the migration distance was shorter, the number of migration cells was lower than that of the control group (P < 0.05), the expression of ZEB1 MMP-2 mRNA and protein was lower than that of the control group (P < 0.05), and the expression of E-cadherin mRNA was lower than that of the control group (P < 0.05). Conclusion the overexpression of miR-429b may inhibit the expression of ZEB1 and MMP-2 in bladder cancer cells, and enhance the expression of E-cadherin to prevent the migration and invasion of bladder cancer cells.
【作者单位】：首都医科大学附属北京潞河医院;
【分类号】：R737.14

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