NAMPT在舌鳞状细胞癌组织中的表达情况及临床意义的相关研究

发布时间：2018-05-01 14:12

本文选题：NAMPT + 舌鳞状细胞癌　；参考：《大连医科大学》2017年硕士论文

【摘要】：目的:舌癌传统的治疗方法对患者口腔颌面部器官的形态及功能破坏大,寻找新的舌癌治疗方式迫在眉睫。本课题旨在探索NAMPT在舌鳞状细胞癌组织中的表达及分布情况,并为舌癌的分子靶向治疗提供依据。材料与方法:1.用免疫组织化学法检测NAMPT在53例舌鳞状细胞癌组织(其中包括40例高分化舌鳞状细胞癌组织、7例中分化舌鳞状细胞癌组织、6例低分化舌鳞状细胞癌组织)及10例癌旁舌组织中的表达及分布情况。2.用蛋白免疫印迹(Western Blot)法检测NAMPT在9例舌鳞状细胞癌组织(其中包括3例高分化舌癌组织及对应癌旁组织,3例中分化舌癌组织及对应癌旁组织,3例低分化舌癌组织及对应癌旁组织)中的表达情况。结果:1.免疫组织化学结果显示:癌旁舌组织中,NAMPT轻微弥散性分布于细胞浆,在部分细胞核中亦见表达。在舌鳞状细胞癌组织中,NAMPT的染色程度均明显增强(P0.01)。另外,在高分化舌癌中,NMAPT在细胞浆中的表达明显增强,在中分化及低分化舌癌中,NAMPT于细胞核、细胞浆中的染色均明显增强。更为重要的是,NAMPT表达量与舌癌分化程度密切相关,与在正常舌组织中的表达量相比,NAMPT在高分化、中分化、低分化舌癌中的表达分别提高到了2.20倍(P0.01)、2.66倍(P0.01)、3.03倍(P0.01)。2.Western Blot结果显示NAMPT在各分化程度的舌癌组织中表达量明显上调(P0.01),与免疫组织化学结果相符合。结论:基于以上数据,我们认为NAMPT在舌癌组织中存在过表达;随着舌癌恶性程度的增加,NAMPT的表达量随之显著增加,本实验数据提示NAMPT可能在舌癌的发生及发展中起到重要作用。随着NAMPT抑制剂在临床抗肿瘤治疗中的不断应用,本实验可能为舌癌的分子靶向治疗提供新的依据。
[Abstract]:Objective: to explore a new treatment for oral and maxillofacial organs in patients with oral and maxillofacial carcinoma. The purpose of this study was to investigate the expression and distribution of NAMPT in tongue squamous cell carcinoma and to provide evidence for molecular targeted therapy of tongue cancer. Materials and methods: 1. Immunohistochemical method was used to detect NAMPT in 53 cases of tongue squamous cell carcinoma (including 40 cases of well-differentiated tongue squamous cell carcinoma 7 cases) and 10 cases of poorly differentiated tongue squamous cell carcinoma (LSCC). Expression and distribution of adjacent tongue. Detection of NAMPT in 9 cases of tongue squamous cell carcinoma (including 3 cases of highly differentiated tongue carcinoma and 3 cases of corresponding paracancerous tissue) by Western blot assay And corresponding tissues adjacent to cancer. The result is 1: 1. Immunohistochemical results showed that NAMPT was slightly distributed in the cytoplasm and expressed in some nuclei of the tongue adjacent to cancer. The degree of NAMPT staining in tongue squamous cell carcinoma was significantly increased (P 0.01). In addition, the expression of NMAPT in the cytoplasm of well differentiated tongue carcinoma was significantly increased, and the expression of napp T in the nucleus and cytoplasm of the well differentiated and poorly differentiated tongue carcinoma were significantly increased. More importantly, the expression of NAMPT was closely related to the degree of tongue cancer differentiation, and it was highly differentiated and moderately differentiated compared with the expression of NAMPT in normal tongue tissue. The expression of NAMPT in poorly differentiated tongue carcinoma was increased to 2.20 times (P 0.01) 2.66 times (P 0.01) and 3.03 times (P 0.01). 2. The results of Western Blot showed that the expression of NAMPT was significantly up-regulated in various differentiated tongue cancer tissues, which was consistent with the immunohistochemical results. Conclusion: based on the above data, we believe that there is overexpression of NAMPT in tongue cancer, and with the increase of malignant degree of tongue cancer, the expression of namp T increases significantly, which suggests that NAMPT may play an important role in the occurrence and development of tongue cancer. With the continuous application of NAMPT inhibitors in clinical antitumor therapy, this study may provide a new basis for molecular targeted therapy of tongue cancer.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R739.86

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