膀胱癌中EYA4基因启动子区甲基化状态及其临床意义的研究

发布时间：2018-05-05 10:41

  本文选题：膀胱癌 + EYA4　； 参考：《安徽医科大学》2017年硕士论文

【摘要】：目的:探讨膀胱癌细胞系和膀胱移行细胞癌组织中EYA4(Eyes Absent 4:EYA转录共激活磷酸酶4)基因启动子区CpG岛甲基化状态及在临床病理特征中的意义。方法:采取甲基化特异性聚合酶链反应(MSP)检测EYA4基因在5株膀胱癌细胞系、1株膀胱永生化上皮细胞系和75例膀胱移行细胞癌组织及癌旁组织中甲基化状态,并结合临床病理特征行统计学分析。结果:EYA4基因启动子区在5株膀胱癌细胞系中,有4株出现甲基化,其甲基化率为80%;在人正常膀胱细胞系中甲基化阴性。EYA4在膀胱癌组织中的甲基化率为70.7%(53/75),癌旁组织中为24%(18/75),差异较为显著,有统计学意义(c2=32.760,P0.01)。EYA4基因启动子区CpG岛甲基化在临床病理特征中如单发膀胱肿瘤与多发膀胱肿瘤相比,直径≤3cm的膀胱癌肿瘤与(29)3cm的膀胱癌肿瘤相比,低级别膀胱肿瘤与高级别膀胱肿瘤相比,浅表性膀胱肿瘤与浸润性膀胱肿瘤相比,差异均有统计学意义(P0.05),但是EYA4基因启动子区CpG岛甲基化程度在与膀胱癌患者的年龄和性别之间差异不明显,无统计学意义(P0.05)。结论:EYA4基因启动子区CpG岛在膀胱癌细胞系和膀胱癌组织中均表现为高度甲基化,推测其可能与膀胱癌的发生和发展有一定的关系,将有助于膀胱癌的早期诊断。EYA4基因启动子区CpG岛区甲基化率与膀胱癌患者的临床病理资料如肿瘤的数目、大小、分级和分期密切相关,与其年龄和性别无相关性。
[Abstract]:Objective: to investigate the methylation status of CpG island in the promoter region of EYA4(Eyes Absent 4:EYA transcription co-activated phosphatase 4 gene in bladder cancer cell line and transitional cell carcinoma (TCC) and its significance in clinicopathological features. Methods: methylation-specific polymerase chain reaction (EYA4) was used to detect the methylation status of EYA4 gene in 5 bladder cancer cell lines, 1 bladder immortalized epithelial cell line and 75 bladder transitional cell carcinoma tissues and adjacent tissues. Combined with clinicopathological features, statistical analysis was made. Results methylation was found in 4 of 5 bladder cancer cell lines. Its methylation rate was 80%; in human normal bladder cell line, methylation negative. EYA4 methylation rate was 70.7% 53% 75% in bladder cancer tissue, and 24% 75% in adjacent tissue, the difference was quite significant. The methylation of CpG island in the promoter region of P0.01. EYA4 gene was statistically significant in the clinicopathological features, such as single bladder tumor compared with multiple bladder tumor, bladder cancer tumor with diameter 鈮，

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