GRP78及COX-2在肝癌组织中的表达及其相关性研究

发布时间：2018-05-05 11:24

本文选题：GRP78 + COX-2　；参考：《安徽医科大学》2017年硕士论文

【摘要】：目的:通过检测原发性肝细胞癌组织中葡萄糖调节蛋白78(glucose-regulated protein 78,GRP78)和环氧合酶-2(Cyclooxygenase-2,COX-2)的表达,分析其相关性与临床病理特征之间的关系。方法:将2001-2007年间在我院病理科证实为肝细胞癌的144例原发性肝癌的患者病理标本制作组织芯片,采用免疫组化法检测组织芯片中GRP78及COX-2的表达情况。结果:1.在144例肝癌组织中GRP78的阳性表达率为47.22%,COX-2的阳性率为56.94%;2.GRP78与乙肝病毒感染病史、谷丙转氨酶(ALT)有显著相关性(P0.05),但与性别、年龄、肝硬化病史、甲胎蛋白(AFP)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、肿瘤大小、分期、分化程度无显著相关性(P0.05)。乙型肝炎病毒表面抗原阳性者中GRP78的阳性表达率为51.23%,谷丙转氨酶(ALT)正常的患者中GRP78的阳性率为59.09%。3.COX-2的表达与肝癌组织分化程度有明显相关性(P0.025),而与性别、年龄、肝硬化病史、乙肝病毒感染史、AFP、AST、ALT、ALP、肿瘤大小、分期无明显相关(P0.05);组织高分化、中分化和低分化的COX-2的阳性率分别为38.63%、59.01%、74.35%。4.相关性分析结果表明在肝癌组织中COX-2与GRP78蛋白的表达有明显的正相关性(r=0.204 P0.025)。结论:1.原发性肝细胞癌组织中GRP78及COX-2均有较高的表达率,表明GRP78可能是肝癌组织内质网应激后续事件中的重要物质,COX-2可能参与肝癌的的发生发展;2.有乙肝病毒感染病史的肝癌患者GRP78表达增高;3.COX-2的表达与肿瘤分化程度有显著相关性,临床上可能作为判断预后的指标之一;4.COX-2的表达与GRP78表达显著正相关,提示在肝癌组织中COX-2与内质网应激存在着密切的关系,肝癌中COX2的表达上调可能是内质网应激凋亡抵抗的重要因素之一。
[Abstract]:Aim: to detect the expression of glucose-regulated protein 78(glucose-regulated protein 78 (GRP78) and cyclooxygenase-2 (COX-2) in primary hepatocellular carcinoma (HCC), and to analyze the relationship between the expression of glucose-regulated protein (78(glucose-regulated protein 78) and the clinicopathological features. Methods: tissue microarray was made from 144 patients with hepatocellular carcinoma (HCC) from 2001 to 2007. The expression of GRP78 and COX-2 in the tissue microarray was detected by immunohistochemical method. The result is 1: 1. The positive rate of GRP78 expression was 56.94 and 56.94 in 144 cases of liver cancer. GRP78 was significantly correlated with history of hepatitis B virus infection and alt (P 0.05), but it was associated with sex, age, and history of cirrhosis. There was no significant correlation among AFP, AST, ALP, tumor size, stage, and differentiation degree (P0. 05%, P 0. 05%, P 0. 05%, P 0. 05%, P 0. 05%, P 0. 05%, P 0. 05%, P 0. 05%). The positive rate of GRP78 in hepatitis B virus surface antigen positive patients was 51.23 and the positive rate of GRP78 in patients with normal alanine aminotransferase (alt) was significantly correlated with the degree of liver cancer differentiation (P0.025), but with sex, age, history of liver cirrhosis. The positive rates of COX-2 with high differentiation, moderate differentiation and low differentiation were 38.63% and 59.01%, respectively. The results of correlation analysis showed that the expression of COX-2 and GRP78 protein was positively correlated with 0.204 P0.025 in HCC. Conclusion 1. The high expression rate of GRP78 and COX-2 in primary hepatocellular carcinoma tissues suggests that GRP78 may be an important substance in the follow-up events of endoplasmic reticulum stress in HCC tissues, and COX-2 may be involved in the occurrence and development of HCC. The expression of COX-2 in HCC patients with history of hepatitis B virus infection was significantly correlated with the degree of tumor differentiation. 4. The expression of COX-2 was positively correlated with the expression of GRP78. It is suggested that there is a close relationship between COX-2 and endoplasmic reticulum stress in hepatocellular carcinoma, and the up-regulation of COX2 expression in HCC may be one of the important factors in resistance to endoplasmic reticulum stress apoptosis.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.7

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