代谢组学法分析骨髓抑制模型小鼠血清小分子代谢产物

发布时间：2018-05-06 20:58

本文选题：气相色谱-质谱 + 代谢组学　；参考：《色谱》2017年12期

【摘要】：骨髓抑制是恶性肿瘤患者化疗后常见的症状,研究骨髓抑制造成体内代谢产物的变化可以为诊断骨髓抑制病症发展状况及采用药物治疗提供思路。采用雄性BalB/C小鼠腹腔注射环磷酰胺建立骨髓抑制模型,通过气相色谱-质谱(GC-MS)对正常与造模小鼠血液代谢产物进行指纹图谱分析,然后采用主成分分析(PCA)和正交偏最小方差判别分析(OPLS-DA)对代谢组学数据进行多维统计分析。与对照组相比,骨髓抑制模型小鼠血浆中潜在的差异表达代谢标志物有15个,其中葡萄糖-1-磷酸、对硝基苯酚、乙酰苯胺、可的松、烟酰胺、马钱苷、咖啡酸、亚油酸和油酸这9种物质的表达差异有统计学意义(P0.05)。结果表明,代谢产物可作为骨髓抑制研究中的重要标记物,有助于揭示化疗所致骨髓抑制的发病机制,判断疾病发展阶段以及后续治疗手段的有效性。
[Abstract]:Bone marrow suppression is a common symptom in patients with malignant tumor after chemotherapy. Studying the changes of metabolites caused by bone marrow suppression can provide ideas for the diagnosis of bone marrow depression and the use of drug therapy. Bone marrow suppression model was established by intraperitoneal injection of cyclophosphamide into male BalB/C mice. The blood metabolites of normal and model mice were analyzed by GC-MS. Then principal component analysis (PCA) and orthogonal partial minimum variance discriminant analysis (OPLS-DAA) were used to analyze the metabolomics data. Compared with the control group, there were 15 potential differentially expressed metabolic markers in the plasma of bone marrow suppression model mice, including glucose-1-phosphate, p-nitrophenol, acetanilide, cortisone, nicotinamide, marganin, caffeic acid. There was significant difference in the expression of linoleic acid and oleic acid between them. The results showed that the metabolites could be used as important markers in the study of bone marrow suppression, which could help to reveal the pathogenesis of chemotherapy-induced bone marrow suppression, and to judge the stage of disease development and the effectiveness of subsequent treatment.
【作者单位】：杭州市第三人民医院肿瘤科;
【基金】：浙江省中医药管理局基金项目(2012ZB131)~~
【分类号】：R730.53

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