鼻咽鳞状细胞癌中PLAC8的表达及临床意义

发布时间：2018-05-07 02:21

本文选题：PLAC + 鼻咽癌(NPC)　；参考：《肿瘤防治研究》2017年07期

【摘要】：目的探讨PLAC8在鼻咽癌组织及体外培养的鼻咽癌细胞中的表达及其与临床病理参数之间的关系。方法应用免疫组织化学染色法检测PLAC8蛋白在51例鼻咽癌组织芯片及32例鼻咽慢性炎性反应组织芯片(TMA)中的表达,分析其与鼻咽癌临床病理参数之间的关系。蛋白印迹法检测PLAC8蛋白在永生化鼻咽上皮细胞系NP69及不同鼻咽癌细胞系(CNE1、CNE2、C666-1、5-8F、6-10B)中的表达情况。结果 PLAC8在51例鼻咽癌组织中表达主要定位于细胞质,表达率为72.5%,在32例鼻咽慢性炎性反应组织中表达率为46.9%,两者间差异有统计学意义(P0.01)。在鼻咽癌组织中PLAC8的表达与T分期、临床分期及远处转移无关(P0.05),而与淋巴结转移有关(P0.05)。蛋白印迹法发现PLAC8蛋白在多株鼻咽癌细胞系中的表达相对于人永生化鼻咽上皮细胞系NP69的表达明显升高。结论 PLAC8可能在鼻咽癌的发生、发展过程中起着重要作用,并可能调控了鼻咽癌的淋巴结转移。
[Abstract]:Objective to investigate the expression of PLAC8 in nasopharyngeal carcinoma (NPC) tissues and in vitro cultured nasopharyngeal carcinoma (NPC) cells and its relationship with clinicopathological parameters. Methods Immunohistochemical staining was used to detect the expression of PLAC8 protein in 51 cases of nasopharyngeal carcinoma and 32 cases of nasopharyngeal chronic inflammatory response tissue microarray. The relationship between the expression of PLAC8 protein and clinicopathological parameters of nasopharyngeal carcinoma was analyzed. The expression of PLAC8 protein was detected by Western blot in immortalized nasopharyngeal epithelial cell line NP69 and different nasopharyngeal carcinoma cell lines CNE1, CNE2C666-1, 5-8FN6-10B. Results the expression of PLAC8 was mainly located in cytoplasm in 51 cases of nasopharyngeal carcinoma, the expression rate was 72.5 and 46.9 in 32 cases of chronic inflammatory reaction of nasopharynx. The difference between the two was statistically significant (P 0.01). The expression of PLAC8 in nasopharyngeal carcinoma was not related to T stage, clinical stage and distant metastasis, but to lymph node metastasis. The expression of PLAC8 protein in many nasopharyngeal carcinoma cell lines was significantly higher than that in human immortalized nasopharyngeal epithelial cell line NP69. Conclusion PLAC8 may play an important role in the pathogenesis and development of nasopharyngeal carcinoma, and may regulate the lymph node metastasis of nasopharyngeal carcinoma.
【作者单位】：武汉大学人民医院耳鼻咽喉头颈外科;
【基金】：国家自然科学基金(81172569)
【分类号】：R739.63

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