Raptor、Rictor与结直肠癌血管生成的相关性及其临床意义

发布时间：2018-05-07 02:31

本文选题：结直肠肿瘤 + Raptor　；参考：《临床与实验病理学杂志》2017年02期

【摘要】：目的检测Raptor、Rictor与血管生成相关因子HIF-1α、HIF-2α以及VEGF在结直肠癌中的表达,探讨Raptor、Rictor与结直肠癌血管生成的相关性及其临床意义。方法采用免疫组化、Western blot、RT-PCR法检测120例结直肠癌组织及60例正常结直肠黏膜组织中Raptor、Rictor及HIF-1α、HIF-2α、VEGF的表达及其差异;CD34标记微血管密度(microvascular density,MVD);分析各指标间的相关性以及与结直肠癌临床病理特征的关系。结果 Raptor、Rictor、HIF-1α、HIF-2α和VEGF蛋白在结直肠癌中的阳性率均明显高于正常结直肠黏膜组织(P0.05)。Raptor、Rictor在中、低分化结直肠癌中的表达高于高分化结直肠癌(P0.05),同时在淋巴结转移组的表达高于无淋巴结转移组(P0.05);HIF-1α、HIF-2α和VEGF在淋巴结转移组的表达高于无淋巴结转移组(P0.05);Raptor、Rictor阳性的结直肠癌组织中MVD明显高于Raptor、Rictor阴性的结直肠癌组织(P0.05);Raptor与HIF-1α、VEGF在结直肠癌中表达呈正相关(P0.01),Rictor与HIF-2α、VEGF表达呈正相关(P0.01),Raptor与Rictor在结直肠癌中表达呈负相关(P0.01)。结论 m TOR核心分子Raptor、Rictor与结直肠癌的发生、发展及血管生成密切相关,两者以不同途径协同促进结直肠癌的血管生成。
[Abstract]:Objective to investigate the relationship between Raptorus Rictor and angiogenesis related factor HIF-1 伪, HIF-2 伪 and VEGF in colorectal cancer and its clinical significance. Methods the expression of RaptorRictor and HIF-1 伪 HIF-2 伪 in 120 cases of colorectal carcinoma and 60 cases of normal colorectal mucosa were detected by immunohistochemistry Western blot RT-PCR, and the difference between microvascular density and microvascular density (MVD) with CD34 was analyzed. Relationship between clinicopathological features of cancer. Results the positive rates of HIF-1 伪 HIF-2 伪 and VEGF protein in colorectal cancer were significantly higher than those in normal colorectal mucosa. The expression of HIF-1 伪 HIF-2 伪 and VEGF in poorly differentiated colorectal carcinoma was higher than that in well-differentiated colorectal carcinoma, and the expression of HIF-1 伪 and VEGF in lymph node metastasis group was higher than that in non-lymph node metastasis group. The expression of P0.05 RaptorRictor positive in lymph node metastasis group was higher than that in non-lymph node metastasis group. There was a positive correlation between the expression of MVD and HIF-1 伪 -VEGF in colorectal cancer tissues, which was significantly higher than that in colorectal cancer tissues with negative Raptor and HIF-1 伪. There was a positive correlation between the expression of MVD and HIF-2 伪 -VEGF in colorectal carcinoma. There was a negative correlation between the expression of MVD and Rictor in colorectal carcinoma. There was a negative correlation between P0.01and Rictor in colorectal cancer. Conclusion Raptorus Rictor, the core molecule of m TOR, is closely related to the occurrence, development and angiogenesis of colorectal cancer. Both of them can promote the angiogenesis of colorectal cancer in different ways.
【作者单位】：滨州医学院附属医院病理科;
【基金】：山东省科技发展计划(2010GSF10259)
【分类号】：R735.34

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