shRNA干扰mPGES-1基因对K562细胞在裸鼠体内成瘤的影响

发布时间：2018-05-07 17:37

本文选题：mPGES- + K细胞　；参考：《中国实验血液学杂志》2017年01期

【摘要】：目的:探讨抑制前列腺素E2合酶1(mPGES-1)表达对人急性白血病K562细胞在裸鼠体内成瘤的影响及其可能的机制。方法:通过shRNA干扰技术下调K562细胞中mPGES-1表达,实验设立了如下研究小组:①干扰组(KD),②阴性细胞(NC)非特异序列shRNA干扰组;③未处理组(CON组);应用Western blot法检测β-catenin和cyclin D1在3组细胞中的表达;构建K562细胞人-裸鼠移植瘤模型,观察移植瘤的生长情况;应用HE染色观察各组肿瘤组织的结构;应用免疫组织化学法检测β-catenin和cyclin D1的表达水平。结果:与CON组和NC组比较,细胞体外实验结果显示KD组β-catenin和cyclin D1表达量减少(P0.05);动物体内实验结果显示,KD组瘤体生长明显减慢,移植瘤体积明显缩小,重量明显减轻(P0.01);HE染色显示,KD组细胞排列相对松散,间质较多,肿瘤细胞核小,细胞质较少;免疫组织化学检测显示,KD组β-catenin和cyclin D1表达量明显下降(P0.05)。结论:下调mPGES-1表达能显著抑制人急性白血病K562细胞在裸鼠体内成瘤,其机制可能与抑制β-catenin和cyclinD1表达相关。
[Abstract]:Aim: to investigate the effect of inhibition of prostaglandin E 2 synthase 1 mPGES-1 expression on tumorigenesis of human acute leukemia K562 cells in nude mice and its possible mechanism. Methods: shRNA interference technique was used to down-regulate the expression of mPGES-1 in K562 cells. The following research group was established as follows: the control group (1: 1) was used to interfere with the non-specific sequence of shRNA interference in K562 cells. 3The expression of 尾 -catenin and cyclin D1 was detected by Western blot method in untreated group, the model of human xenograft tumor of K562 cells was established, the growth of transplanted tumor was observed, the structure of tumor tissue was observed by HE staining, and the expression of 尾 -catenin and cyclin D1 were detected by HE staining. The expression levels of 尾-catenin and cyclin D1 were detected by immunohistochemistry. Results: compared with CON group and NC group, the expression of 尾 -catenin and cyclin D1 in KD group was decreased in vitro, and the growth of tumor and the volume of transplanted tumor in KD group were significantly decreased in vivo. The expression of 尾 -catenin and cyclin D1 in KD group was significantly decreased by immunohistochemical staining, and the expression of 尾 -catenin and cyclin D1 was significantly decreased in KD group. Conclusion: down-regulation of mPGES-1 expression can significantly inhibit the tumorigenesis of human acute leukemia K562 cells in nude mice, and its mechanism may be related to the inhibition of 尾 -catenin and cyclinD1 expression.
【作者单位】：中山大学附属孙逸仙纪念医院血液内科;
【基金】：2012年国家自然青年科学基金(81200342) 2012年广东省医学科学技术研究基金(A2012182) 2014年度广东省公益研究与能力建设专项基金项目(2014A020212085)
【分类号】：R733.7

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