新型光敏剂叶绿光1号介导的光动力治疗合用血管内皮生长因子抑制剂在胆管癌细胞系FRH0201中作用的实验研究

发布时间：2018-05-08 00:12

本文选题：光动力治疗(PDT) + 胆管癌　；参考：《第二军医大学》2015年硕士论文

【摘要】：目的：对于无法手术切除的胆管癌病人,光动力治疗展现出了很好的应用前景。我们的研究目的是评估YLG-I作为光敏剂在光动力治疗中的有效性以及YLG-I介导的光动力治疗合用Avastin是否能增益光动力治疗在胆管癌细胞系FRH0201中的作用。方法：本研究以人胆管癌细胞系FRH0201为研究对象,在体外实验中应用新型光敏剂YLG-I对FRH0201进行光动力治疗处理,652nm波长激发后,观察细胞的生长情况,并通过CCK8实验比较对照组、Avastin组、光动力治疗组及光动力治疗加Avastin组之间肿瘤细胞的生长抑制情况。体内实验中,建立裸鼠皮下移植瘤模型,实施光动力治疗后比较对照组、Avastin组、光动力治疗组及光动力治疗加Avastin组之间裸鼠皮下肿瘤的生长情况。留取皮下移植瘤标本,做免疫组织化学染色分析各组肿瘤组织内VEGF的表达情况。结果：YLG-I介导的光动力治疗组和YLG-I介导的光动力治疗组合用Avastin组与空白对照组及单独使用Avastin治疗组比较在肿瘤治疗效果上有着极为明显的获益。在合用Avastin治疗组中可以观察到血管内皮生长因子的表达受到了明显的抑制。结论：YLG-I作为光敏剂在光动力治疗中的应用有着很好的有效性,同时合用血管内皮生长因子抑制剂以阻断血管内皮生长因子通路能够减少VEGF的表达或许是增益光动力治疗疗效的有效途径。
[Abstract]:Objective: photodynamic therapy is promising for cholangiocarcinoma patients who can not be resected surgically. Our aim was to evaluate the efficacy of YLG-I as a Guang Min agent in photodynamic therapy and whether YLG-I mediated photodynamic therapy combined with Avastin could enhance the role of photodynamic therapy in cholangiocarcinoma cell line FRH0201. Methods: in this study, the human cholangiocarcinoma cell line FRH0201 was studied. In vitro, a new Guang Min agent, YLG-I, was used to treat FRH0201 with photodynamic therapy at 652 nm wavelength. The growth of the cells was observed, and the control group was compared with the control group by CCK8 experiment. Growth inhibition of tumor cells between photodynamic therapy group and photodynamic therapy plus Avastin group. In vivo model of subcutaneous transplanted tumor was established in nude mice. After photodynamic therapy the growth of subcutaneous tumor in nude mice was compared among control group photodynamic therapy group photodynamic therapy group and photodynamic therapy plus Avastin group. The expression of VEGF in tumor tissues was analyzed by immunohistochemical staining. Results compared with the control group and the Avastin alone group, the Avastin group and the YLG-I mediated photodynamic therapy group were significantly beneficial in the treatment of tumor. The expression of vascular endothelial growth factor (VEGF) was significantly inhibited in Avastin treatment group. Conclusion as Guang Min, the application of WYLG-I in photodynamic therapy is very effective. At the same time, the combination of vascular endothelial growth factor inhibitor to block the vascular endothelial growth factor pathway can reduce the expression of VEGF may be an effective way to gain photodynamic therapy.
【学位授予单位】：第二军医大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R735.8

【共引文献】