被转化生长因子β活化的长链非编码RNA对人淋巴瘤Raji细胞增殖、周期及凋亡的影响

发布时间：2018-05-08 22:03

本文选题：被转化生长因子β活化的长链非编码RNA + Raji细胞　；参考：《中国现代医学杂志》2017年05期

【摘要】：目的研究被转化生长因子β活化的长链非编码RNA(LncRNA-ATB)对人淋巴瘤Raji细胞增殖、凋亡和周期的影响及其潜在机制。方法通过实时荧光定量PCR(qRT-PCR)检测LncRNA-ATB短发夹RNA(short hairpin RNA,shRNA)和miR-200c模拟物(miR-200c mimics)效果,以及LncR NA-ATB、miR-200c和鼠类肉瘤病毒癌基因(KRAS)的表达;噻唑蓝(MTT)法检测沉默LncRNA-ATB对Raji细胞增殖的影响;流式细胞术检测LncR NA-ATB对Raji细胞凋亡和周期的影响;荧光素酶报告基因(Luciferase)实验检测KRAS是否为miR-200c的直接靶基因。结果 LncR NA-ATB-shRNA可有效沉默Raji细胞中LncRNAATB的表达,沉默LncRNA-ATB的表达可抑制Raji细胞的增殖能力,促进Raji细胞的凋亡能力,并使Raji细胞的周期阻滞于G_1期。进一步研究显示LncRNA-ATB可作为ceRNA与miR-200c竞争性地调控miR-200c直接靶分子KRAS的表达而促进肿瘤细胞增殖。结论LncRNA-ATB可通过上调KRAS的表达而在淋巴瘤细胞的增殖、凋亡和周期过程中发挥着重要的作用。
[Abstract]:Objective to investigate the effects of LncRNA-ATB) activated by transforming growth factor 尾 (TGF- 尾) on the proliferation, apoptosis and cell cycle of human lymphoma Raji cells and its potential mechanism. Methods the effects of LncRNA-ATB short hairpin RNA(short hairpin RNA-shRNAs and miR-200c mimics, as well as the expression of LncR NA-ATBmmiR-200c and murine sarcoma virus oncogene (KRAS) were detected by real-time fluorescence quantitative PCR, and the effects of LncRNA-ATB silencing on the proliferation of Raji cells were detected by MTT assay. Flow cytometry was used to detect the effect of LncR NA-ATB on apoptosis and cell cycle of Raji cells, and luciferase reporter gene (luciferase) was used to detect whether KRAS was a direct target gene of miR-200c. Results LncR NA-ATB-shRNA could effectively silence the expression of LncRNAATB in Raji cells, and silence the expression of LncRNA-ATB could inhibit the proliferation of Raji cells, promote the apoptosis of Raji cells, and block the Raji cell cycle in G1 phase. Further studies have shown that LncRNA-ATB can be used as ceRNA and miR-200c to competitively regulate the expression of KRAS, a direct target molecule of miR-200c, and promote the proliferation of tumor cells. Conclusion LncRNA-ATB may play an important role in the proliferation, apoptosis and cell cycle of lymphoma cells by upregulating the expression of KRAS.
【作者单位】：西南医科大学;西南医科大学附属医院血液内科;
【分类号】：R733.1

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