小檗碱对肺癌干细胞增殖和凋亡的影响及其机制

发布时间：2018-05-09 00:07

本文选题：肺肿瘤 + 肿瘤干细胞　；参考：《中国组织工程研究》2017年09期

【摘要】：背景:前期研究发现小檗碱对多种肿瘤和肿瘤干细胞具有抑制作用,但是对肺癌干细胞的影响报道较少。目的:探讨小檗碱对肺癌干细胞增殖和凋亡的影响及其可能的机制。方法:采用免疫磁珠法分离CD133~+肺癌干细胞,MTT法和流式细胞仪分别检测不同质量浓度小檗碱(0,2.5,5,10,20,40 mg/L)对CD133~+肺癌干细胞增殖和凋亡的影响,免疫印迹检测小檗碱(0,20 mg/L)对肺癌干细胞中Ki67、Bax、Bcl-2、PTCH1、SHH、Gli-1和SMO蛋白表达的影响。结果与结论:(1)采用免疫磁珠法分选得到高比率的CD133+肺癌干细胞;(2)小檗碱抑制肺癌干细胞生长,并呈浓度依赖性;(3)小檗碱促进肺癌干细胞凋亡,并呈浓度依赖性;(4)Ki67、Bcl-2、PTCH1、SHH、Gli-1和SMO蛋白在20 mg/L小檗碱组中的表达水平明显低于未加药组,Bax蛋白的表达水平则明显高于未加药组;(5)结果表明,小檗碱可能通过调控Hedgehog信号通路抑制肺癌干细胞增殖,促进其凋亡。
[Abstract]:Background: previous studies have shown that berberine can inhibit many kinds of tumor and tumor stem cells, but the effect on lung cancer stem cells is less reported. Objective: to investigate the effect of berberine on proliferation and apoptosis of lung cancer stem cells and its possible mechanism. Methods: MTT assay and flow cytometry were used to detect the effects of different concentrations of berberine on proliferation and apoptosis of CD133- lung cancer stem cells. The effect of berberine 20 mg / L on the expression of Ki67 Bax-Bcl-2PTCH1 / SHHli-1 and SMO protein in lung cancer stem cells was detected by Western blot. Results and conclusion: a high percentage of CD133 lung cancer stem cells were obtained by immunomagnetic bead sorting. Berberine inhibited the growth of lung cancer stem cells in a concentration-dependent manner, and berberine promoted the apoptosis of lung cancer stem cells. The expression levels of Bcl-2PTCH1 and SMO proteins in the 20 mg/L berberine group were significantly lower than those in the control group (P < 0.01). The results showed that the expression level of Bcl-2PTCH1 and SMO protein in the 20 mg/L berberine group was significantly higher than that in the control group (P < 0.05). Berberine may inhibit the proliferation and apoptosis of lung cancer stem cells by regulating Hedgehog signaling pathway.
【作者单位】：南阳市中心医院病理科;
【分类号】：R734.2

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