钌配合物对肝癌BEL-7402细胞生长影响的研究

发布时间：2018-05-09 08:53

本文选题：钌配合物 + 肝癌细胞　；参考：《山西医科大学》2017年硕士论文

【摘要】：目的:在全球经济蓬勃发展的背景下,随着医疗水平的逐步提高,肿瘤的发病率和检测率同时也在逐年的提高,肿瘤仍然是当今社会人类健康的一个重大威胁。近几年来各国学者对于原发性肝癌的发病机制及其治疗效果取得了许多重大的研究成果,但其生存率依然较低,预后仍差的现象一直无法取得新的进展,因此寻求新的治疗药物与治疗方法具有重要意义。化疗药物在当下治疗肿瘤的研究中已经取得很大的发展,同时也为原发性肝癌的治疗提供了新的思路。诸多文献均有报道,钌配合物能够抑制肿瘤细胞的生长,观察钌配合药物对肝癌细胞生长的抑制作用,从而为临床工作提供新的治疗依据。方法:实验室培养肝癌BEL-7402细胞,将培养的细胞随机分为A、B两组,A组为空白对照组,B组中加入钌配合药物Ru-HMIP,使用MTT法测定细胞增殖的抑制率,用流式细胞术测定细胞的凋亡率。结果:1.MTT法检测结果示:同对照组对比,钌配合药物Ru-HMIP对肝癌细胞生长具有抑制作用,抑制率大于对照组。2.流式细胞仪检测结果显示:钌配合药物Ru-HMIP作用的肝癌细胞,肝癌细胞凋亡明显增多。结论:在肝癌BEL-7402细胞生长过程中,钌配合药物对其具有抑制作用。
[Abstract]:Objective: with the rapid development of the global economy, with the gradual improvement of the medical level, the incidence and detection rate of tumor are also increasing year by year. Cancer is still a major threat to human health in today's society. In recent years, many scholars have made a lot of significant effects on the pathogenesis and treatment effect of primary liver cancer. However, the survival rate is still low and the prognosis is still poor, so it is of great significance to seek new therapeutic drugs and treatment methods. Chemotherapy drugs have made great progress in the current research of cancer treatment, and also provide new ideas for the treatment of primary liver cancer. It is reported that ruthenium complexes can inhibit the growth of tumor cells and observe the inhibitory effect of ruthenium combined with drugs on the growth of hepatoma cells, thus providing new therapeutic basis for clinical work. Methods: the BEL-7402 cells of liver cancer were cultured in laboratory. The cultured cells were randomly divided into A, B two groups, A group as blank control group, and B group joined ruthenium. The inhibitory rate of cell proliferation was measured by MTT method and the apoptosis rate of cells was measured by flow cytometry. Results: the results of 1.MTT assay showed that ruthenium combined with drug Ru-HMIP had inhibitory effect on the growth of hepatoma cells compared with the control group, and the inhibition rate was greater than that of the control group of.2. flow cytometer: ruthenium combined with drug Ru-HMIP. The apoptosis of HCC cells increased significantly. Conclusion: Ru combined with drugs can inhibit the growth of BEL-7402 cells.

【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.7

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