低氧对肺癌A549细胞血管因子水平的影响

发布时间：2018-05-14 02:02

本文选题：低氧 + 肺癌　；参考：《青海大学》2017年硕士论文

【摘要】：目的:观察低氧对肺癌A549细胞血管因子的分泌以及细胞移行能力的影响。方法:(1)将肺癌A549细胞分为实验组和对照组,实验组细胞培养于低氧环境中,对照组细胞培养于常氧环境中。(2)分别在实验组和对照组的12孔板接种2×105个细胞,于培养72小时后收集细胞的上清液,用ELISA法检测上清液中HIF-1α、VEGF、TNF-α、ANG-2、TGF-α及TGF-β1的表达水平。(3)进行划痕实验,分别于培养0小时、6小时、20小时后在光镜下观察实验组和对照组的细胞的移行情况。结果:(1)与对照组比较,实验组细胞上清中的HIF-1α、VEGF、TNF-α、Ang-2水平均高于对照组,差异有统计学意义(p0.05),实验组TGF-α、TGF-β1的水平均低于对照组,差异有统计学意义(p0.05),Ang-2实验组与对照组比较,差异无统计学意义(p0.05)。(2)与对照组比较,实验组培养0小时、6小时、20小时的细胞移行速度加快,划痕的覆盖时间缩短。结论:(1)低氧可以调控A549细胞分泌的多种血管因子的表达水平,从而参与肺癌的发病机制。HIF-1α等信号通路通过各种方式对某些血管因子有一定的调控作用。(2)肺癌A549细胞在低氧微环境中较常氧具有更强的移行能力,低氧环境能促进肺癌细胞的增殖。
[Abstract]:Aim: to observe the effect of hypoxia on the secretion of vascular factor and cell migration ability of lung cancer A549 cells. Methods: lung cancer A549 cells were divided into experimental group and control group. The experimental group cells were cultured in hypoxic environment, and the control group cells were cultured in normoxic environment. 2 脳 105 cells were inoculated on the 12-well plate of experimental group and control group, respectively. After 72 hours of culture, the supernatants of the cells were collected, and the expression levels of HIF-1 伪 -VEGF- TNF- 伪 and TGF- 尾 1 in the supernatants were detected by ELISA method. The expression levels of TGF- 伪 and TGF- 尾 1 in the supernatants were detected by scratch assay. The migration of cells in the experimental group and the control group were observed under light microscope after 0 hours, 6 hours and 20 hours of culture, respectively. Results compared with the control group, the levels of HIF-1 伪 -VEGF- 伪 TNF- 伪 Ang-2 in the supernatant of the experimental group were higher than those in the control group (P 0.05), and the levels of TGF- 伪 TGF- 尾 1 in the experimental group were lower than those in the control group, and the difference was statistically significant between the experimental group and the control group. There was no significant difference between the two groups. Compared with the control group, the cell migration rate of the experimental group was accelerated and the covering time of the scratch was shortened after 0 hours or 6 hours or 20 hours of culture. Conclusion hypoxia can regulate the expression of many vascular factors secreted by A549 cells. Thus, the signaling pathways such as HIF-1 伪 involved in the pathogenesis of lung cancer can regulate some vascular factors in a variety of ways) the lung cancer A549 cells have stronger translocation ability than normal oxygen in hypoxic microenvironment. Hypoxia can promote the proliferation of lung cancer cells.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R734.2

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