FOXC2对肺腺癌细胞恶性增殖及侵袭迁移能力的影响

发布时间：2018-05-14 06:12

本文选题：叉头框C蛋白 + 肺腺癌　；参考：《中国老年学杂志》2017年24期

【摘要】：目的探究叉头框C2蛋白(FOXC2)对肺腺癌细胞恶性增殖及侵袭迁移能力的影响。方法利用慢病毒转染的方法在人肺腺癌细胞系A549中构建过表达FOXC2细胞(实验组)及相应对照细胞系(对照组),Western印迹检测两组细胞FOXC2及干性相关分子Oct4、Sox2及Nanog的表达水平,MTT法检测两组细胞0、24、48、72、96 h的恶性增殖能力,Transwell侵袭及迁移实验检测两组细胞侵袭迁移能力。结果与对照组细胞比较,实验组细胞FOXC2、Oct4、Sox2及Nanog表达水平均显著上调(P0.05),细胞恶性增殖能力在48、72及96 h出现显著升高(P0.05),细胞侵袭及迁移能力均显著上调(P0.05),差异均有统计学意义。结论 FOXC2可促进肺腺癌细胞恶性增殖及侵袭迁移能力,其机制与介导肿瘤细胞干细胞特性形成有关。
[Abstract]:Objective to investigate the effects of forked C2 protein (FOXC2) on the proliferation and invasion and migration of lung adenocarcinoma cells. Methods the expression of FOXC2 cells (experimental group) and corresponding control cell lines (control group) in human lung adenocarcinoma cell line A549 were constructed by lentivirus transfection. The Western blot was used to detect FOXC2 and Oct4 in two groups of cells. The expression level of Sox2 and Nanog, MTT method was used to detect the malignant proliferation ability of 0,24,48,72,96 h in two groups of cells. Transwell invasion and migration test was used to detect the invasion and migration of cells in the two groups. Results compared with the control group, the expression level of FOXC2, Oct4, Sox2 and Nanog in the experimental group were up (P0.05), and the cell malignant proliferation ability was in 48,72 and 96 Significant increase (P0.05), cell invasion and migration ability were significantly up (P0.05), and the difference was statistically significant. Conclusion FOXC2 can promote the malignant proliferation and invasion and migration of lung adenocarcinoma cells, and the mechanism is related to the formation of tumor cell stem cell characteristics.

【作者单位】：遵义医学院附属医院呼吸内科;遵义医学院附属医院肿瘤科;
【分类号】：R734.2

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