ATRA与BMP9对人骨肉瘤细胞增殖及分化作用的研究
本文选题：骨肉瘤 + 全反式维甲酸 ； 参考：《第三军医大学学报》2017年13期
【摘要】：目的研究全反式维甲酸(all-trans-retinoic acid,ATRA)与骨形态发生蛋白9(bone morphogenetic proteins 9,BMP9)对人骨肉瘤细胞增殖及分化的影响。方法利用四甲基偶氮唑蓝(MTT)法和流式细胞仪分析细胞增殖情况;蛋白质免疫印迹实验(Western blot)检测细胞增殖和成骨相关指标;半定量PCR及Western blot分析ATRA和ATRA与BMP9联用处理143B细胞株后对BMP9表达水平的影响。结果在人骨肉瘤143B细胞株中,与对照组相比,ATRA能明显抑制细胞增殖(P0.05),并促使细胞周期阻滞在G1期(P0.05),下调PCNA的蛋白水平,并呈浓度依赖性;ATRA能呈浓度依赖性增加晚期成骨指标OCN和OPN的蛋白水平(P0.05);ATRA能上调BMP9的m RNA及蛋白水平,并呈浓度依赖性(P0.05);单用BMP9并不能上调OCN和OPN表达,并促进其增殖(P0.05),但与ATRA联用时,能够增强ATRA诱导的相关成骨指标表达和抗增殖作用(P0.05)。结论ATRA对143B细胞的增殖具有抑制作用并诱导其成骨分化,并可能恢复BMP9的成骨诱导能力。
[Abstract]:Objective to study the effects of all-trans-retinoic acid ATRAA and bone morphogenetic protein 9(bone morphogenetic proteins 9 on proliferation and differentiation of human osteosarcoma cells. Methods the cell proliferation was analyzed by MTT and flow cytometry, and the cell proliferation and osteoblast related indexes were detected by Western blot assay. The effect of ATRA, ATRA and BMP9 on the expression of BMP9 in 143B cell line was analyzed by semi-quantitative PCR and Western blot. Results in human osteosarcoma cell line 143B, compared with the control group, PCNA could significantly inhibit the proliferation of human osteosarcoma cell line P0.05A, promote cell cycle arrest at G1 phase, and down-regulate the protein level of PCNA. In a concentration-dependent manner, ATRA could increase the protein levels of OCN and OPN in a concentration-dependent manner. ATRA could upregulate m RNA and protein levels of BMP9 in a concentration-dependent manner, but BMP9 alone could not up-regulate the expression of OCN and OPN. In combination with ATRA, the expression of osteogenic markers induced by ATRA and the anti-proliferative effect of P0.05 could be enhanced. Conclusion ATRA can inhibit the proliferation of 143B cells and induce the osteogenic differentiation of 143B cells, and may restore the osteogenic ability of BMP9.
【作者单位】： 重庆医科大学附属第二医院骨科;重庆市生物化学与分子药理学重点实验室;重庆医科大学药学院药理学教研室;
【基金】：国家自然科学基金面上项目(81572226)~~
【分类号】：R738
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