白藜芦醇靶向EGFR和c-Met信号通路抑制非小细胞肺癌细胞增殖的机制

发布时间：2018-05-14 09:42

本文选题：非小细胞肺癌 + 白藜芦醇　；参考：《肿瘤防治研究》2017年01期

【摘要】：目的探讨白藜芦醇抑制非小细胞肺癌细胞增殖的分子机制。方法非小细胞肺癌细胞用白藜芦醇处理24、48和72 h,MTS和软琼脂集落形成实验检测白藜芦醇对非小细胞肺癌的抑制作用,免疫印迹检测白藜芦醇对表皮生长因子受体(epidermal growth factor receptor,EGFR)和肝细胞生长因子受体(c-Met)表达水平及下游信号通路的影响,流式细胞术检测白藜芦醇对细胞周期的影响。结果白藜芦醇剂量依赖性抑制非小细胞肺癌细胞的增殖。白藜芦醇抑制EGFR和c-Met信号通路磷酸化活化的同时下调了EGFR总蛋白及EGFR在胞膜和胞核的表达,同时降低了c-Met蛋白在胞膜的表达及c-Met蛋白60 kD大小剪切体在胞核的表达。白藜芦醇促进了A549细胞G_0/G_1期阻滞。结论白藜芦醇通过靶向EGFR和c-Met信号通路抑制非小细胞肺癌细胞的增殖。
[Abstract]:Objective to investigate the molecular mechanism of resveratrol to inhibit the proliferation of non small cell lung cancer cells. Methods non small cell lung cancer cells were treated with resveratrol for 24,48 and 72 h, MTS and soft agar colony formation to detect the inhibitory effect of resveratrol on non small cell lung cancer. The effect of wth factor receptor, EGFR) and hepatocyte growth factor receptor (c-Met) expression level and downstream signal pathway. Flow cytometry tests the effect of resveratrol on cell cycle. Results resveratrol inhibits the proliferation of non small cell lung cancer cells in a dose dependent manner. Resveratrol inhibits the activation of phosphorylation of EGFR and c-Met signaling pathways. The expression of EGFR protein and EGFR in the cell membrane and nucleus were downregulated, and the expression of c-Met protein in the cell membrane and the expression of c-Met protein 60 kD shear body in the nucleus were reduced. Resveratrol promoted the G_0/G_1 phase block of A549 cells. Conclusion resveratrol inhibited the proliferation of non small cell lung cancer cells by targeting EGFR and c-Met signaling pathway.

【作者单位】：中南大学附属湘雅二医院胸心外科;中南大学附属湘雅三医院放射科;湖南省肿瘤医院中南大学湘雅医学院附属肿瘤医院病理科;
【基金】：国家自然科学基金(81401548)
【分类号】：R734.2

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