ANO1、EGFR在食管上皮异型增生组织和癌变进程中的表达及其意义

发布时间：2018-05-15 00:34

本文选题：ANO + EGFR　；参考：《中国病原生物学杂志》2017年05期

【摘要】：目的比较钙离子激活氯离子通道蛋白1(anoctamin 1,ANO1)和表皮生长因子受体(epidermal growth factor receptor,EGFR)蛋白在小鼠不同食管上皮组织病变中的表达,探讨ANO1、EGFR蛋白表达与小鼠食管上皮病变的关系,为早期食管癌变诊断提供参考。方法应用化学致癌剂4-硝基喹啉-1-氧化物(4NQO)由饮水法建立小鼠食管癌前病变模型,HE染色光镜下比较观察食管正常组织、轻度、中度、重度异型增生组织及癌变组织变化,采用免疫组化法检测小鼠食管不同病变组织中EGFR、ANO1蛋白表达情况。结果至实验第24周末,共收集小鼠食管正常组织14份、异型增生组织23份(其中轻度异型增生7份、中度异型增生8份、重度异型增生8份)、癌变组织18份。其中ANO1和EGFR蛋白在食管轻度异型增生组织、中度异型增生组织和正常组织中的阳性率分别为0(0/7)、12.50%(1/8)、0(0/14)和14.29%(1/7)、12.50%(1/8)、7.14%(1/14),差异均无统计学意义(P0.05);ANO1和EGFR在癌组织中的阳性率分别为55.56%(10/18)和61.11%(11/18),与ANO1和EGFR在异型增生组织(轻度、中度和重度增生组织)中的阳性率17.39%(4/23)和26.09%(6/23)及其正常组织中的阳性率0(0/14)和7.14%(1/14)比较差异有统计学意义(P0.05);ANO1和EGFR在重度异型增生组织中的阳性率分别为37.50%(3/8)和50%(4/8),与正常组织比较差异有统计学意义(P0.05)。在18份食管癌组织中,ANO1阳性组EGFR阳性率90%(9/10),ANO1阴性组EGFR阴性率75%(6/8),两种蛋白的表达呈正相关(r=0.663,P=0.003)。结论 ANO1、EGFR蛋白在小鼠食管癌组织及重度异型增生上皮组织中存在过表达,且两种蛋白在食管癌组织中的表达呈正相关,提示ANO1和EGFR两者有可能成为食管癌早期诊断的生物学标志物。
[Abstract]:Objective to compare the expression of calcium activated chloride channel protein (1(anoctamin 1) and epidermal growth factor receptor (EGFR) protein in different esophageal epithelial lesions in mice, and to explore the relationship between the expression of ANO1 + EGFR protein and mouse esophageal epithelial lesions. To provide reference for early diagnosis of esophageal carcinoma. Methods using the chemical carcinogen 4-nitroquinoline-1-oxide-4NQO to establish mouse model of esophageal precancerous lesion by drinking water, the changes of normal esophageal tissues, mild, moderate and severe dysplasia and cancerous tissues were observed under light microscope. The expression of EGFR- ANO1 protein in different tissues of mouse esophagus was detected by immunohistochemical method. Results by the end of the 24th week of the experiment, 14 normal tissues of mouse esophagus were collected, 23 of them were dysplasia (7 cases of mild dysplasia, 8 cases of moderate dysplasia, 8 cases of severe dysplasia, 18 cases of cancerous tissue). ANO1 and EGFR proteins were found in mild dysplasia of esophagus. The positive rates in moderate dysplasia tissues and normal tissues were 0 / 7 and 12.50%, respectively, and 14.2929 / 1 / 7% 12.50% 12.50% and 7.14%, respectively. There was no significant difference in the positive rates of P0.05ANO1 and EGFR in cancer tissues (55.556 / 1018) and 61.11111118% (P < 0.05) and 61.111118% (P < 0.05), respectively. In moderate and severe hyperplastic tissues, the positive rates were 17.39% 4 / 23) and 26.09% 6 / 23) and the positive rates in normal tissues were 0 / 14) and 7.14% / 14) respectively. The positive rates of ANO1 and EGFR in severe dysplasia tissues were 37.50 / 3 / 8 and 50 / 4 / 8 respectively, which were significantly higher than those in normal tissues. The difference was statistically significant (P 0.05). In 18 cases of esophageal carcinoma, the positive rate of EGFR in the positive group of ANO1 was 90% and the negative rate of EGFR was 75% / 8% in the negative group of ANO1. The expression of the two proteins was positively correlated with the expression of 0.663P (0.003%). Conclusion ANO1-EGFR protein is overexpressed in mouse esophageal carcinoma and severe dysplasia epithelium, and the expression of ANO1-EGFR protein is positively related to the expression of ANO1-EGFR protein in esophageal carcinoma. The results suggest that ANO1 and EGFR may be biomarkers for early diagnosis of esophageal carcinoma.
【作者单位】：山东大学医学院;济宁医学院诊断学教研室;山东省医学科学院山东省寄生虫病防治研究所;
【基金】：济宁市科技局资助项目(No.2015130)
【分类号】：R735.1

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