高通量测序分析结肠腺瘤患者肠道黏膜菌群的构成变化

发布时间：2018-05-16 01:31

本文选题：结肠腺瘤 + 肠道菌群　；参考：《南方医科大学学报》2017年09期

【摘要】：目的探讨结直肠癌发展早期肠道菌群的变化规律。方法通过结肠镜活检收集31例结肠腺瘤患者的腺瘤组织以及20例健康志愿者的肠道黏膜,提取基因组DNA,对细菌16SrRNA基因序列V_3~V_4高变区进行PCR扩增,lluminaMiSeq平台上高通量测序分析。结果结肠腺瘤患者的肠道黏膜菌群多样性比健康对照人群显著增高(P0.01),腺瘤组黏膜菌群结构和健康对照者相比差异显著。门水平上:结肠腺瘤组变形菌门明显增多(P0.01),厚壁菌门/拟杆菌门比值下降。属水平上:结肠腺瘤组乳球菌属、链球菌属和假单胞菌属增多(P0.01),而肠球菌属,杆菌属丰度明显下降(P0.01)。不同病理类型腺瘤比较发现:结肠高级别和低级别上皮内瘤变组肠道黏膜菌群整体结构相似,埃希菌-志贺菌属在高级别上皮内瘤变组呈现上升趋势,但差异无统计学意义(P0.05)。结论结肠腺瘤患者肠道菌群多样性和构成与正常健康人存在明显差异,腺瘤患者黏膜菌群结构发生明显失衡,形成了易于肿瘤生长的肠道微生态环境。
[Abstract]:Objective to investigate the changes of intestinal flora in the early stage of colorectal cancer development. Methods the adenoma tissues of 31 patients with colonic adenoma and the intestinal mucosa of 20 healthy volunteers were collected by colonoscopy biopsy. The genomic DNA was extracted and the high throughput sequencing was performed on the V_3~V_4 hypervariable region of bacterial 16SrRNA gene sequence on the platform of Lumina MiSeq. Results the diversity of intestinal mucosal flora in patients with colonic adenoma was significantly higher than that in healthy controls (P 0.01), and the structure of mucosal flora in adenoma was significantly higher than that in healthy controls. At the level of portal, the phylum of Proteus in colonic adenoma group increased significantly (P 0.01), and the ratio of phylum thuringiensis to Bacteroides decreased. The genus of Lactococcus, Streptococcus and Pseudomonas in colonic adenoma group increased P0.01G, while the abundance of Bacillus decreased significantly in Enterococcus. The comparison of adenomas of different pathological types showed that the whole structure of intestinal mucosal flora was similar in the high grade and low grade intraepithelial neoplasia groups, and that the Escherichia coli and Shigella showed an upward trend in the high grade intraepithelial neoplasia group, but the difference was not statistically significant (P 0.05). Conclusion the diversity and composition of intestinal flora in patients with colonic adenoma are significantly different from those in normal controls. The mucosal flora of adenoma patients is out of balance and the intestinal microecological environment is easy to grow.
【作者单位】：南京医科大学附属上海一院临床医学院消化内科;
【基金】：国家自然科学基金(81600500) 上海交通大学医工交叉基金(YG2014ZD10)~~
【分类号】：R735.35

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