乳腺浸润性导管癌17号染色体拷贝数变异与临床病理特征相关性分析

发布时间：2018-05-18 04:27

  本文选题：乳腺癌 + 号染色体　； 参考：《中华肿瘤防治杂志》2017年12期

【摘要】：目的乳腺癌是影响妇女健康的主要癌症之一,且发病率呈现明显上升趋势。DNA拷贝数变化(copy number variation,CNV)能够影响基因表达,从而引起肿瘤发生,研究CNV对探索肿瘤的发病机制有很大帮助。本研究探讨乳腺癌17号染色体拷贝数变异和临床病理特征关系。方法应用荧光原位杂交(fluorescence in situ hybridization,FISH)法检测2010-01-01-2011-01-01新疆医科大学附属肿瘤医院乳腺润性导管癌标本283例,观察17号染色体拷贝数情况及人表皮生长因子受体2(human epidermal growth factor receptor 2,HER2)基因扩增情况,并用免疫组织化学法检测HER2受体蛋白的表达水平。结果 283例乳腺癌中17号染色体拷贝数多倍体为43例(15.19%),双倍体为226例(79.86%),单倍体为14例(4.95%),乳腺癌17号染色体多倍体HER2基因阳性率为53.49%(23/43)。283例乳腺癌中HER2基因表达阳性为84例,阳性率为29.68%(84/283);17号染色体拷贝数不同在HER2基因是否扩增方面差异有统计学意义,χ~2=9.564,P=0.007;乳腺癌17号染色体拷贝数变异在p53基因表达(χ~2=8.181,P=0.017)、病理组织学分级(χ~2=11.203,P=0.019)方面差异有统计学意义,与年龄、肿瘤大小和淋巴结转移等指标差异无统计学意义。17号染色体拷贝数不同组中预后存在差异,差异有统计学意义,χ~2=241.363,P=0.001。结论 17号染色体拷贝数变异和乳腺癌病理特征及预后相关,检测乳腺癌中17号染色体拷贝数变异可作为指导临床治疗和判断预后的参考指标。
[Abstract]:Objective Breast cancer is one of the major cancers affecting women's health, and the incidence of breast cancer is obviously increasing. Copy number variation can affect gene expression and cause tumorigenesis. The study of CNV is of great help in exploring the pathogenesis of tumor. This study was to investigate the relationship between chromosome 17 copy number variation and clinicopathological features of breast cancer. Methods fluorescence in situ hybridization (fish) method was used to detect 283 breast ductal carcinoma samples from Xinjiang Medical University affiliated Cancer Hospital from January to January 2011. The copy number of chromosome 17 and the amplification of human epidermal growth factor receptor 2(human epidermal growth factor receptor 2HER2 gene were observed. The expression of HER2 receptor protein was detected by immunohistochemistry. Results among 283 cases of breast cancer, 43 cases were polyploid of chromosome 17 copy number, 226 cases were diploidy, 14 cases were haploid, and the positive rate of polyploid HER2 gene on chromosome 17 was 53.49%. The positive rate of HER2 gene was 84 cases in 23 / 43 / 283 cases of breast cancer. The positive rate of chromosome 17 was 29.68% / 283%; the number of copies of chromosome 17 was significantly different in the amplification of HER2 gene, 蠂 ~ 2 / 9.564 / P ~ (0.007); the variation of chromosome 17 copy number of breast cancer was significantly different in the expression of p53 gene (蠂 ~ (2) (蠂 ~ (2) = 8.181), histopathological grade (蠂 ~ (211.203) P ~ (0.019). There was no significant difference in tumor size and lymph node metastasis, but there was significant difference in prognosis among the groups with different copy number of chromosome 17, 蠂 ~ (2 +) 241.363 (P ~ (0.001). Conclusion the variation of chromosome 17 copy number is related to the pathological characteristics and prognosis of breast cancer. Detection of variation of chromosome 17 copy number in breast cancer can be used as a reference index for clinical treatment and prognosis.
【作者单位】： 新疆医科大学附属肿瘤医院乳腺外科一病区;
【基金】：乌鲁木齐市科学技术计划(Y151310041)
【分类号】：R737.9
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本文编号：1904378