PAX2和C-MYC在子宫内膜癌组织中的表达及其意义

发布时间：2018-05-19 23:11

本文选题：PAX2 + C-MYC　；参考：《右江民族医学院》2017年硕士论文

【摘要】：目的:探讨PAX2与C-MYC在子宫内膜癌中发生发展的作用,进一步分析子宫内膜癌中PAX2及C-MYC表达与临床病理参数的关系,为子宫内膜癌机制研究和临床早期诊断提供新的思路。方法:收集2014年10月至2016年10月右江民族医学院附属医院和附属河池医院手术切除子宫内膜癌组织60例、不典型增生组织35例、正常子宫内膜组织35例;采用免疫组化(IHC)检测PAX2及C-MYC在三种不同子宫内膜中蛋白表达情况;从以上标本中留取新鲜子宫内膜癌组织30例、不典型增生组织12例、正常子宫内膜组织15例;应用实时荧光定量聚合酶链反应(Quantitative Real-time PCR,Q-PCR)检测PAX2与C-MYC m RNA在子宫内膜癌、不典型增生内膜及正常子宫内膜组织中表达。所有数据采用SPSS17软件进行分析,以P0.05认为具有统计学意义。结果:(1)免疫组化(IHC):PAX2在正常子宫内膜组、不典型子宫内膜组、子宫内膜癌组阳性表达率分别为74.29%,48.57%,43.3%,三组之间阳性表达率差异具有统计学意义(P0.05)。PAX2在正常子宫内膜组阳性率高于不典型增生组(P0.05)和子宫内膜癌组(P0.01);子宫内膜癌组阳性表达率低于不典型增生组,差异无统计学意义(P0.05)。PAX2蛋白表达与子宫内膜癌组织的病理分化程度(P0.05)、肌层浸润深度相关(P0.05),与淋巴结转移、临床分期、年龄、病理分型无关(P0.05)。C-MYC蛋白在正常子宫内膜组、不典型增生组、子宫内膜癌组阳性表达率分别为17.14%,40.0%,66.67%,三组间阳性表达率差异具有统计学意义(P0.05)。C-MYC在子宫内膜癌组中的阳性表达率高于正常内膜组(P0.01)和不典型增生内膜组(P0.05),不典型增生内膜组中阳性率高于正常内膜组(P0.01)。C-MYC蛋白水平表达与子宫内膜癌患者的病理分化(P0.05)、手术-临床分期(P0.05)密切相关;与肌层浸润深度、淋巴结转移、年龄、病理分型无关(P0.05)。(2)实时荧光定量PCR(Q-PCR):PAX2 mRNA在不同子宫内膜组织中表达量总体有差异(P0.01)。PAX2mRNA在子宫内膜癌组中表达水平低于正常子宫内膜对照组(P0.01);不典型增生组PAX2 mRNA低于正常子宫内膜组(P0.01);子宫内膜癌组低于不典型增生内膜组(P0.05)。C-MYC mRNA在不同子宫内膜组织中表达水平总体有差异(P0.001)。C-MYC m RNA在正常子宫内膜组表达水平高于子宫内膜癌组(P0.01)、不典型增生内膜组(P0.01),子宫内膜癌组中的mRNA表达高于不典型增生内膜组(P0.01)。结论:(1)PAX2在不典型增生组织、子宫内膜癌组织中的表达低于正常子宫内膜组织,提示PAX2表达下调可能是子宫内膜不良病变的早期事件。(2)C-MYC在子宫内膜癌组织中表达上调,可能促进了子宫内膜癌的发生发展,C-MYC有可能成为子宫内膜癌潜在的诊断和治疗靶点。
[Abstract]:Objective: to investigate the role of PAX2 and C-MYC in the occurrence and development of endometrial carcinoma, and to further analyze the relationship between the expression of PAX2 and C-MYC and clinicopathological parameters in endometrial carcinoma, so as to provide a new idea for the study of mechanism and early clinical diagnosis of endometrial carcinoma. Methods: from October 2014 to October 2016, 60 cases of endometrial carcinoma, 35 cases of atypical hyperplasia and 35 cases of normal endometrium were collected from affiliated Hospital and affiliated Hechi Hospital of Youjiang National Medical College. The expression of PAX2 and C-MYC in three kinds of endometrium were detected by immunohistochemistry, 30 cases of fresh endometrial carcinoma, 12 cases of atypical hyperplasia and 15 cases of normal endometrium were collected from above samples. The expression of PAX2 and C-MYC m RNA in endometrial carcinoma, atypical hyperplasia and normal endometrium was detected by real-time quantitative Real-time PCR Q-PCR. All the data were analyzed by SPSS17 software, and P0.05 was considered statistically significant. Results in the normal endometrium group and the atypical endometrium group, the immunohistochemical staining showed that IHC: PAX2 was found in the normal endometrium group and the atypical endometrium group. The positive rate of positive expression in endometrial carcinoma group was 74.29 and 48.57 and 43.3 respectively. The positive expression rate of P0.05U. PAX2 in normal endometrium group was significantly higher than that in atypical hyperplasia group (P0.05) and endometrial carcinoma group (P0.01a). The expression rate was lower than that in atypical hyperplasia group. There was no significant difference between the expression of P0.05, PAX2 protein and the degree of pathological differentiation of endometrial carcinoma. The depth of myometrium invasion was correlated with lymph node metastasis, clinical stage, age and pathological classification. There was no significant difference between P0.05 and C-MYC protein in normal endometrium. Atypical hyperplasia group, The positive expression rates of P0.05, C-MYC in endometrial carcinoma group were significantly higher than those in normal endometrial carcinoma group (P 0.01) and atypical hyperplasia endometrium group (P 0.05%, P 0.05%). The positive rate in endometrial group was higher than that in normal endometrium group. The expression of C-MYC protein was closely related to the pathological differentiation of endometrial carcinoma patients (P0.05, operation-clinical stage, P0.05). Depth of myometrial invasion, lymph node metastasis, age, The expression of real-time quantitative PCR(Q-PCR):PAX2 mRNA in different endometrial tissues was lower than that in normal endometrial carcinoma group (P 0.01) and PAX2 mRNA in atypical hyperplasia group was lower than that in normal endometrium control group (P 0.01), while the expression of P0.01 + PAX2 mRNA in endometrial carcinoma group was lower than that in normal endometrial carcinoma group (P < 0.05), while that in atypical hyperplasia group was lower than that in normal endometrial tissue. The expression of P0. 05 mRNA in endometrial carcinoma group was lower than that in atypical hyperplasia endometrium group. There was a general difference in the expression level of P0. 001. C-MYC m RNA in different endometrial tissues. The expression level of C-MYC m RNA in normal endometrium group was higher than that in endometrial carcinoma group (P0. 01). The expression of mRNA in endometrial carcinoma group was higher than that in atypical hyperplasia group. Conclusion the expression of PAX2 in atypical hyperplasia and endometrial carcinoma is lower than that in normal endometrial tissue, suggesting that the down-regulation of PAX2 expression may be an early event of adverse endometrial lesions. C-MYC may be a potential target for the diagnosis and treatment of endometrial carcinoma.
【学位授予单位】：右江民族医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.33

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