信号素4D在结直肠癌组织中的表达及其与MVD的相关性

发布时间：2018-05-21 16:17

本文选题：信号素4D + 结直肠癌　；参考：《昆明医科大学》2017年硕士论文

【摘要】：[目的]探讨结直肠癌组织中信号素4D (Semaphorin4D,Sema4D)的表达及其与微血管密度(Microvessel density, MVD)的相关性。[方法]回顾性分析2014-2015年间在云南省肿瘤医院结直肠外科接受结直肠癌根治性手术的61例患者的临床病理资料;男性38例,女性23例,中位年龄55岁;采用免疫组织化学SP法检测Semma4D蛋白在61例结直肠癌组织和56例正常肠粘膜组织(距癌组织边缘5cm以上)中的表达水平;用免疫组化SP法检测CD34标记的微血管并计数微血管密度。[结果]Sema4D蛋白主要在结直肠癌组织的细胞膜及细胞质内表达,在正常肠粘膜组织细胞质内呈阴性表达。Sema4D在癌组织的阳性表达率[100%(61/61)]明显高于正常肠粘膜组织[0(0/56)],差异有统计学意义(X 2=113.027, P0.001)。结直肠癌组织中Sema4D与MVD两者作相关性分析,Spearman相关系数r=0.333, p=0.009,两者呈现明显的正相关性;MVD表达量在Sema4D表达的高、低组之间作t检验分析,p=0.003 (p0.01),差异具有显著的统计学意义。[结论]Sema4D在结直肠癌组织内高水平表达,且与MVD存在明显的正相关性,MVD随着Sema4D表达的升高而增加,提示Sema4D有望成为潜在的结直肠癌血管治疗靶点。
[Abstract]:[objective] to investigate the expression of semaphorin 4 D in colorectal carcinoma and its correlation with microvessel density (MVD). [methods] the clinicopathological data of 61 patients undergoing radical colorectal cancer surgery in the Department of Colorectal surgery in Yunnan Oncology Hospital from 2014 to 2015 were retrospectively analyzed, including 38 males and 23 females with median age of 55 years. Immunohistochemical SP method was used to detect the expression of Semma4D protein in 61 cases of colorectal carcinoma and 56 cases of normal intestinal mucosa (more than 5cm from the edge of cancer tissue), the microvessel labeled by CD34 was detected by SP immunohistochemical method and the microvessel density was counted. [results] Sema4D protein was mainly expressed in the cell membrane and cytoplasm of colorectal cancer. The positive rate of negative expression of Sema4D in the cytoplasm of normal intestinal mucosa was significantly higher than that in normal intestinal mucosa [100% 61 / 61] and normal intestinal mucosa [0 / 56]. The difference was statistically significant (P 0.001). Correlation Analysis of Sema4D and MVD in Colorectal Cancer; Spearman correlation coefficient r = 0.333, p = 0.009. There was a significant positive correlation between Sema4D and MVD in the expression of Sema4D, and a significant difference was found between the two groups by t test. [conclusion] there is a high level of Sema4D expression in colorectal cancer tissues, and there is a significant positive correlation between MVD and MVD, which suggests that Sema4D may be a potential target for vascular therapy for colorectal cancer.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.34

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