SOX4对非小细胞肺癌细胞A549的顺铂耐药作用的影响

发布时间：2018-05-22 07:52

本文选题：肺肿瘤 + A　；参考：《中国肺癌杂志》2017年05期

【摘要】：背景与目的肺癌是最严重的恶性肿瘤之一,其中非小细胞肺癌发病率在肺癌中居首位。部分患者对顺铂耐受是导致化疗失败的主要原因。SOX4是转录调节因子,在多种肿瘤的发生发展过程中发挥着重要的作用,通过调控β-catenin的表达对Wnt信号通路进行调控。本研究旨在探讨SOX4对非小细胞肺癌细胞A549的顺铂耐药作用的影响。方法体外诱导法建立顺铂耐药的肺癌细胞株A549/DDP,CCK8法检测A549及A549/DDP细胞对顺铂的耐药性。绘制A549与A549/DDP细胞生长曲线。Western blot检测耐药细胞株中SOX4的蛋白表达水平。CCK8法检测敲减SOX4后耐药细胞株A549/DDP对顺铂耐药性。实时定量PCR和免疫印迹法检测敲减SOX4后A549/DPP细胞中β-catenin和Survivin蛋白的表达。结果成功构建非小细胞肺癌顺铂耐药细胞株A549/DDP,其耐药性是亲本细胞的13.7倍,差异有统计学意义(P0.001)。生长曲线显示耐药细胞株与亲本细胞增殖速度没有统计学差异(P0.05);与A549细胞相比,耐药细胞A549/DDP细胞中SOX4的表达显著增高(P0.001)。敲减SOX4后,A549/DDP细胞的耐药性显著降低;敲减SOX4后,A549/DDP细胞中β-catenin和Survivin的m RNA和蛋白表达水平显著降低。结论 SOX4的表达水平影响非小细胞肺癌细胞A549对顺铂的耐药性。
[Abstract]:Background & objective Lung cancer is one of the most serious malignant tumors. In some patients, cisplatin tolerance is the main cause of chemotherapy failure. SOX4 is a transcriptional regulator, which plays an important role in the occurrence and development of many kinds of tumors. It regulates the Wnt signaling pathway by regulating the expression of 尾 -catenin. The aim of this study was to investigate the effect of SOX4 on cisplatin resistance of non-small cell lung cancer cell line A549. Methods Cisplatin resistant lung cancer cell line A549 / DDP- CCK8 was established by in vitro induction method to detect the resistance of A549 and A549/DDP cells to cisplatin. The growth curve of A549 and A549/DDP cell line. Western blot was used to detect the expression of SOX4 protein in drug resistant cell line. CCK8 method was used to detect the resistance of A549/DDP to cisplatin after knocking down SOX4. The expression of 尾 -catenin and Survivin in A549/DPP cells after knockout was detected by real-time quantitative PCR and Western blotting. Results A 549% DDP resistant cell line of non-small cell lung cancer (NSCLC) was successfully constructed and its resistance was 13.7 times as much as that of parent cell. The difference was statistically significant (P 0.001). The growth curve showed that there was no significant difference in proliferation rate between drug resistant cell line and parent cell line P0. 05%. Compared with A549 cell line, the expression of SOX4 in drug resistant cell line A549/DDP cells was significantly higher than that in A549 cell line (P 0. 001). The drug resistance of A549 / DDP cells decreased significantly after knockout of SOX4, and the m RNA and protein expression of 尾 -catenin and Survivin in A549 / DDP cells after knockout was significantly decreased. Conclusion the expression of SOX4 affects the resistance of non-small cell lung cancer cell line A549 to cisplatin.
【作者单位】：天津中医药大学第一附属医院检验科;天津医科大学总医院肿瘤科;
【分类号】：R734.2

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