二甲双胍对卵巢癌细胞侵袭迁移及MTA1表达的影响

发布时间：2018-05-25 01:44

本文选题：卵巢癌 + 二甲双胍　；参考：《现代妇产科进展》2017年04期

【摘要】：目的:体外细胞培养实验检测二甲双胍对卵巢癌细胞SKOV3侵袭迁移能力的影响,以及其对转移相关因子1(MTA1)表达的影响。方法:用不同浓度二甲双胍处理卵巢癌细胞,采用划痕实验和transwell实验检测二甲双胍对细胞侵袭迁移能力的影响,Western blot法检测二甲双胍对细胞中MTA1蛋白表达的影响,qRT-PCR检测二甲双胍对细胞中MTA1 mRNA表达的影响。结果:与对照组(0mmol/L)相比,二甲双胍干预组的细胞迁移速度明显下降(P0.01);作用24h时,2.5mmol/L与5mmol/L组未见差异(P0.05);作用48h时,卵巢癌细胞的迁移速度随着二甲双胍浓度的增加而减弱(P0.01)。随着二甲双胍浓度的增加,穿膜细胞数减少(P0.01);二甲双胍干预组的MTA1 mRNA及MTA1蛋白表达明显降低(P0.05),不同药物浓度之间无差异。结论:二甲双胍抑制SKOV3细胞侵袭迁移,抑制作用与浓度呈正相关,其机制可能与抑制MTA1表达有关。
[Abstract]:Aim: to investigate the effect of metformin on the invasion and migration of ovarian cancer cell line SKOV3 and the expression of metastasis related factor 1 (MTA1) in vitro. Methods: ovarian cancer cells were treated with metformin at different concentrations. The effect of metformin on the ability of cell invasion and migration was detected by scratch test and transwell assay. The effect of metformin on the expression of MTA1 protein was detected by Western blot. The effect of metformin on the expression of MTA1 mRNA was detected by qRT-PCR. Results: compared with the control group (0 mmol / L), the cell migration rate of metformin group decreased significantly (P 0.01), and there was no difference between 5mmol/L group and 2. 5 mmol / L group at 24 h, and the migration rate of ovarian cancer cells decreased with the increase of metformin concentration at 48 h. With the increase of metformin concentration, the number of transmembrane cells decreased, while the expression of MTA1 mRNA and MTA1 protein decreased significantly in metformin intervention group, and there was no difference among different drug concentrations. Conclusion: metformin inhibits the invasion and migration of SKOV3 cells, and the inhibitory effect is positively correlated with the concentration. The mechanism may be related to the inhibition of MTA1 expression.
【作者单位】：华北理工大学研究生院;华北理工大学附属医院妇产科;
【分类号】：R737.31

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