CXC型趋化因子配体16在乳腺浸润性导管癌中的表达研究

发布时间：2018-05-26 02:51

本文选题：乳腺浸润性导管癌 + CXCL16　；参考：《昆明医科大学》2017年硕士论文

【摘要】：目的:本实验通过检测乳腺浸润性导管癌患者的癌组织及癌旁组织中CXCL16蛋白的浓度,比较二者之间的差异。同时探讨乳腺浸润性导管癌患者的临床特征与癌组织中表达的CXCL16蛋白浓度之间的关系。方法:本实验研究对象为2015年6月至2016年8月于昆明医科大学第一附属医院乳腺外科诊治,穿刺病理确诊为乳腺浸润性导管癌并手术,术后石蜡报告确诊乳腺浸润性导管癌患者40例,分别收集癌组织、癌旁组织,分为2组:实验组(癌组织40例),对照组(癌旁组织40例)。采用酶联免疫吸附(ELISA)的实验方法,分别测定实验组及对照组中CXCL16蛋白的浓度,分析其浓度与乳腺浸润性导管癌之间的相关性。根据癌组织中CXCL16蛋白的浓度分析其与乳腺浸润性导管癌不同的TNM分期、复发转移危险度分级、淋巴结是否转移、分子分型、年龄、绝经与否、乳腺炎病史等临床特征之间是否存在相关性。实验数据采用SPSS19.0统计学软件进行统计分析,统计数据分析采用方差分析和t检验。结果:1.CXCL16蛋白在乳腺浸润性导管癌患者的癌组织中的表达明显高于癌旁组织,且表达存在显著的统计学差异(P=0.0019)。2.CXCL16蛋白的表达在有乳腺炎病史的乳腺浸润性导管癌患者中的表达明显高于无乳腺炎病史的患者,且表达存在显著的统计学差异(P=0.000)。3.CXCL16蛋白在TNM分期越高、复发转移危险度越高、有淋巴结转移的乳腺浸润性导管癌患者中的表达高于低TNM分期、低复发转移危险度及无淋巴结转移的患者,且表达存在统计学差异(P值0.05)。4.CXCL16蛋白的表达在不同分子亚型、不同年龄、绝经与否的乳腺浸润性导管癌患者中无统计学差异(P值0.05)。结论:1.CXCL16蛋白的浓度在乳腺浸润性导管癌患者的癌组织中明显高于癌旁组织,提示CXCL16蛋白与乳腺浸润性导管癌之间存在相关性。2.CXCL16蛋白的表达在有乳腺炎病史的乳腺浸润性导管癌患者的癌组织中较无乳腺炎病史的患者高,提示既往有乳腺炎病史的患者患乳腺癌的机率可能增加。3.乳腺浸润性导管癌患者的癌组织中CXCL16蛋白的浓度与乳腺癌的TNM分期、复发转移危险度分级、淋巴结转移间存在相关性,随着乳腺浸润型导管癌的恶性程度增高,CXCL16蛋白的浓度升高,其可能作为乳腺癌恶性程度、浸润转移的评估指标之一,且对其预后有一定的辅助预测作用。4.乳腺浸润性导管癌患者的癌组织中的CXCL16蛋白表达与患者年龄、绝经与否、不同的分子分型之间未发现相关性。
[Abstract]:Aim: to detect the concentration of CXCL16 protein in breast infiltrating ductal carcinoma and its adjacent tissues. At the same time, the relationship between the clinical features of breast invasive ductal carcinoma and the expression of CXCL16 protein in breast cancer tissue was investigated. Methods: from June 2015 to August 2016, breast surgery was performed in the first affiliated Hospital of Kunming Medical University. Forty cases of breast invasive ductal carcinoma were diagnosed by paraffin wax after operation, and were collected and divided into two groups: the experimental group (40 cases) and the control group (40 cases). The concentration of CXCL16 protein in the experimental group and the control group was determined by Elisa and the correlation between the concentration and the invasive ductal carcinoma of breast was analyzed. According to the concentration of CXCL16 protein in cancer tissues, the TNM staging, the risk of recurrence and metastasis, lymph node metastasis, molecular type, age, menopause or not were analyzed. Whether there is a correlation between clinical features such as mastitis history. The experimental data were analyzed by SPSS19.0 software, and the statistical data were analyzed by ANOVA and t test. Results 1. The expression of CXCL16 protein in breast invasive ductal carcinoma was significantly higher than that in adjacent tissues. The expression of CXCL16 protein in breast infiltrating ductal carcinoma with history of mastitis was significantly higher than that in patients with no history of mastitis. The higher the TNM stage, the higher the risk of recurrence and metastasis. The higher the expression of CXCL16 protein in breast invasive ductal carcinoma with lymph node metastasis was, the higher the expression of CXCL16 protein was in breast invasive ductal carcinoma with lymph node metastasis. In patients with low risk of recurrence and metastasis and no lymph node metastasis, there was no significant difference in the expression of P value 0.05).4.CXCL16 protein in patients with breast invasive ductal carcinoma with different molecular subtypes, age, menopause or not. Conclusion the concentration of CXCL16 protein in breast invasive ductal carcinoma is significantly higher than that in adjacent tissues. 2. The expression of CXCL16 protein in breast invasive ductal carcinoma with history of mastitis was higher than that in patients with no history of mastitis, suggesting that the expression of CXCL16 protein in breast invasive ductal carcinoma was significantly higher than that in breast invasive ductal carcinoma with history of mastitis. The results suggest that patients with previous history of mastitis may have an increased risk of breast cancer. The concentration of CXCL16 protein in breast invasive ductal carcinoma was correlated with TNM stage, risk of recurrence and metastasis, lymph node metastasis. With the increase of malignant degree of invasive ductal carcinoma of breast, CXCL16 protein may be used as one of the evaluation indexes of malignant degree, invasion and metastasis of breast cancer, and it may be helpful to predict the prognosis of breast cancer. There was no correlation between the expression of CXCL16 protein and age, menopause or not, and different molecular typing in breast invasive ductal carcinoma.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.9

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