生长抑制因子4基因在子宫内膜癌中的表达及意义

发布时间：2018-05-27 02:26

本文选题：生长抑制因子4基因 + 子宫内膜癌　；参考：《郑州大学》2015年硕士论文

【摘要】：背景及目的:子宫内膜癌是一组由子宫内膜恶变形成的恶性肿瘤,是女性生殖系统中的三大恶性肿瘤之一,其组织病理类型中以子宫内膜样腺癌最为常见,目前,临床期别较早的子宫内膜癌治愈率较高,但是,对于临床期别较晚、伴有局部复发或远处转移的患者,其治愈率仍较低,故早发现、早治疗对子宫内膜癌尤为重要。区别于宫颈癌,子宫内膜癌缺少早期筛查方法,且由于取材局限或误诊为月经失调,其早期诊断率较低。因此探索、研究新的检测指标在子宫内膜癌的发生、发展、浸润及转移中的作用机制,将对子宫内膜癌的早期临床诊断、预后评价产生极大的指导意义。生长抑制因子家族是近年来发现的一组较为重要的肿瘤生长抑制因子,已有大量研究证明其与体内多个系统肿瘤的发生有关。近些年的研究发现,ING4基因在多种肿瘤组织中都有表达缺失,但是在子宫内膜癌中ING4的研究为空白,本研究旨在测定生长抑制因子4(ING4)基因在不同病理子宫内膜中的表达,并研究其表达与子宫内膜癌发生、发展及预后的关系。方法:采用免疫荧光PCR方法和免疫组化S-P法检测25例正常的增生期子宫内膜、15例不典型增生的子宫内膜及45例子宫内膜样腺癌组织中ING4的表达情况,观察结果并进行统计学处理。结果:免疫组化S-P法结果显示ING4在正常增生期的子宫内膜中表达最多,不典型增生子宫内膜组织中的表达量显著低于正常增生期子宫内膜组织,子宫内膜样腺癌组织中的表达量最低,3组的比较差异有统计学意义(P0.05)。免疫荧光PCR结果与免疫组化法测定的结果相一致,ING4 m RNA的相对表达量在正常增生期的子宫内膜组织、不典型增生的子宫内膜组织、子宫内膜样腺癌的组织中逐渐降低,统计学分析显示,各组间差异有统计学意义(P0.05);在子宫内膜样腺癌组织中ING4的表达与组织学分级显著相关(P0.05),与肌层浸润深度、淋巴结转移无关(P0.05)。但其表达与组织-病理学分期的关系尚需进一步研究。结论:ING4在子宫内膜癌和不典型增生中的表达有不同程度的丢失,ING4的基因突变或表达异常甚至缺失可能参与启动了子宫内膜癌的恶变过程。检测ING4可能成为子宫内膜癌早期诊断及预测癌前病变癌变风险重要指标之一。
[Abstract]:Background and objective: endometrial carcinoma is a group of malignant neoplasms formed by endometrial malignancy. It is one of the three major malignant tumors in the female reproductive system. Endometrial adenocarcinoma is the most common histopathological type of endometrial carcinoma. The cure rate of early stage endometrial carcinoma is higher, but for the patients with local recurrence or distant metastasis, the cure rate is still low, so early detection, early treatment is particularly important for endometrial carcinoma. Different from cervical cancer, endometrial carcinoma lacks early screening method, and its early diagnosis rate is low because of limited material taken or misdiagnosed as menstrual disorder. Therefore, to explore the role of new detection indicators in the occurrence, development, invasion and metastasis of endometrial carcinoma will be of great significance for the early clinical diagnosis and prognosis evaluation of endometrial carcinoma. The growth inhibitory factor family is a group of important tumor growth suppressor found in recent years, which has been proved to be related to the occurrence of multiple systemic tumors in vivo by a large number of studies. In recent years, it has been found that the expression of ING4 gene is absent in many kinds of tumor tissues, but the study of ING4 in endometrial carcinoma is blank. The purpose of this study is to determine the expression of growth inhibitor 4 (NG4) gene in different pathological endometrium. To study the relationship between its expression and the occurrence, development and prognosis of endometrial carcinoma. Methods: the expression of ING4 in 25 cases of normal proliferative endometrium and 45 cases of endometrioid adenocarcinoma were detected by immunofluorescence PCR method and S-P immunohistochemical method in 15 cases of atypical hyperplasia of endometrium and 45 cases of endometrial adenocarcinoma. The results were observed and statistically analyzed. Results: the results of S-P immunohistochemical staining showed that the expression of ING4 was the most in the endometrium of normal proliferative phase, and the expression of ING4 in atypical hyperplasia endometrium was significantly lower than that in normal proliferative endometrium. There was significant difference among the three groups in the lowest expression of endometrial adenocarcinoma (P 0.05). The relative expression of ING4 m RNA in endometrial tissues of normal proliferative phase, atypical hyperplasia of endometrium and endometrial adenocarcinoma was decreased gradually by immunofluorescence PCR and immunohistochemical method, and the expression of ING4 m RNA in endometrial tissues of normal proliferative phase, atypical hyperplasia of endometrium and endometrial adenocarcinoma was decreased gradually. Statistical analysis showed that there were significant differences among the three groups (P 0.05), and the expression of ING4 in endometrial adenocarcinoma was significantly correlated with histological grade (P 0.05), but not with the depth of myometrial invasion and lymph node metastasis (P 0.05). However, the relationship between its expression and histopathological staging needs further study. Conclusion the gene mutation or abnormal expression of ING4 in endometrial carcinoma and atypical hyperplasia may be involved in the carcinogenesis of endometrial carcinoma. Detection of ING4 may be one of the important markers for early diagnosis of endometrial carcinoma and prediction of the risk of precancerous lesions.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R737.33

【参考文献】