流式细胞术检测结直肠癌根治术前后循环肿瘤细胞和循环肿瘤干细胞及其临床预测价值

发布时间：2018-05-27 06:25

本文选题：结直肠癌 + 循环肿瘤细胞　；参考：《中国肿瘤生物治疗杂志》2017年04期

【摘要】：目的:探讨流式细胞仪检测结直肠癌根治术前后循环肿瘤细胞(circulating tumor cell,CTC)和循环肿瘤干细胞(circulating tumor stem cell,CTSC)作为患者临床预测指标的可行性和临床价值。方法:入组首诊初治50例结直肠癌患者,手术前后各抽取15 ml外周血。分离单个核细胞后分成两份,一份标记CTC标志物(CD45、Ep CAM和CK),另一份标记CTSC标志物(CD45、Ep CAM、CD44和CD133),Moflo XDP流式细胞仪对其进行检测,CD45-Ep CAM~+CK~+细胞确定为CTC;CTSC确定为3群:CD44~+CTSC、CD133~+CTSC和CD44~+CD133~+CTSC。结果:结直肠癌根治术前患者CTC和CD44~+CTSC阳性率分别为34%和44%,CD133~+CTSC和CD44~+CD133~+CTSC是24%和0;术后患者CTC和CD44~+CTSC阳性率分别为38%和54%,CD133~+CTSC和CD44~+CD133~+CTSC分别为26%和0。根治术前后CTC阳性率都与肿瘤T分期有关联(P0.05);术后CTC与肿瘤的浸润深度、淋巴结转移和远处转移有关联(P0.05);术前CD44~+CTSC与肿瘤的浸润深度有关联。结论:根治术前后CTC及术前CD44~+CTSC阳性率都具有临床预测指标的可行性和价值,术后CTC阳性率可能是更好的预测指标。
[Abstract]:Objective: to investigate the feasibility and clinical value of flow cytometry in detecting circulating tumor cell CTCs and circulating tumor stem cells before and after radical resection of colorectal cancer. Methods: 50 patients with colorectal cancer were treated for the first time, and 15 ml peripheral blood was collected before and after operation. After isolation, mononuclear cells were divided into two groups: one labeled CTC marker CD45Ep CAM and CKP, the other labeled CTSC marker CD45EpCAMfCD44 and CD133OMoflo XDP flow cytometry. The cells were identified as three groups of CD44 ~ CTSC CD133 ~ CTSC and CD44 ~ CD133 ~ CTSCC by flow cytometry (FCM). The results showed that the mononuclear cells were classified into three groups: CD44 ~ CTSCT CD133 ~ CTSC and CD44 ~ CD133 ~ CTSC.The results showed that the mononuclear cells were classified into three groups: CD44- CTSC-CD133- CTSC and CD44- CD133- CTSCS. Results: the positive rates of CTC and CD44 ~ CTSC in patients with colorectal cancer before radical operation were 34% and 44%, respectively, and the positive rates of CTC and CD44 ~ CTSC were 24% and 0, respectively, and the positive rates of CTC and CD44 ~ CTSC were 38% and 54% respectively, and the positive rates of CD44 ~ CD133 ~ CTSC were 26% and 0 respectively. The positive rate of CTC was correlated with T stage of tumor before and after radical operation, CTC was correlated with depth of invasion, lymph node metastasis and distant metastasis after operation, and CD44 ~ CTSC was correlated with depth of invasion before operation. Conclusion: the positive rate of CTC before and after radical operation and the positive rate of CD44 ~ CTSC before and after operation have the feasibility and value of clinical prediction. The positive rate of CTC after operation may be a better predictor.
【作者单位】：福建医科大学附属肿瘤医院福建省肿瘤医院国家临床重点肿瘤专科福建省肿瘤生物治疗重点实验室;
【基金】：福建省医学创新基金资助项目(No.2015-CXB-4) 福建省自然科学基金资助项目(No.2016J01436) 福建省卫教联合项目(No.WKJ-FJ-14) 国家临床重点专科建设基金资助项目(No.2013-544)~~
【分类号】：R735.3;;R730.43;;R730.7

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