他莫昔芬与辛伐他汀对人乳腺癌细胞MCF-7的协同效应及机制

发布时间：2018-05-27 11:00

本文选题：他莫昔芬 + 辛伐他汀　；参考：《安徽医科大学学报》2017年05期

【摘要】：目的探究他莫昔芬(TAM)与辛伐他汀(SIV)对人乳腺癌细胞株(MCF-7)的增殖抑制作用及其潜在的作用机制。方法 MTT检测TAM、SIV单独以及联合使用对MCF-7细胞株的增殖抑制作用,计算TAM与SIV单独作用时IC50值以及联合使用时对细胞作用的联合指数(CI),评价两药之间的相互作用。荧光显微镜下观察并拍摄各组中的细胞凋亡形态学变化。流式细胞术检测TAM组、SIV组、TAM+SIV组药物处理后的MCF-7细胞凋亡情况。Western blot法检测各组细胞Bcl-2、Bax的表达情况。结果 MTT法表明,TAM与SIV单药对MCF-7有增殖抑制作用,联合作用时抑制更为明显,且呈协同作用;荧光显微镜及流式细胞术显示TAM与SIV单药对MCF-7有诱导凋亡作用,联合时作用增强;Western blot法表明TAM+SIV组中Bcl-2表达量较细胞对照组及TAM组、SIV组下降而Bax表达量增加且更加明显。结论 TAM与SIV联合使用时可显著抑制MCF-7细胞株的增殖,同时可明显诱导MCF-7细胞株凋亡,提示两药联合使用时,乳腺癌患者可能有更好的获益。
[Abstract]:Objective to investigate the inhibitory effect of tamoxifen (TAM) and simvastatin (SIV) on the proliferation of human breast cancer cell line MCF-7 and its possible mechanism. Methods MTT was used to detect the inhibitory effect of Tammy SIV on the proliferation of MCF-7 cell line. The IC50 value of TAM and SIV alone and the combined index of TAM and SIV were calculated to evaluate the interaction between the two drugs. The morphological changes of apoptosis were observed and photographed under fluorescence microscope. Flow cytometry was used to detect the apoptosis of MCF-7 cells treated with Tam SIV in TAM group. Western blot assay was used to detect the expression of Bcl-2P Bax. Results the results of MTT showed that Tam and SIV could inhibit the proliferation of MCF-7, and the inhibition was more obvious and synergistic. Fluorescence microscope and flow cytometry showed that TAM and SIV could induce apoptosis of MCF-7. The expression of Bcl-2 in the TAM SIV group was lower than that in the control group and TAM group, but the expression of Bax in the TAM SIV group was significantly higher than that in the control group and TAM group. Conclusion the combination of TAM and SIV can significantly inhibit the proliferation of MCF-7 cell line and induce the apoptosis of MCF-7 cell line, suggesting that the combination of the two drugs may benefit breast cancer patients better.
【作者单位】：安徽医科大学第一附属医院肿瘤科;合肥市滨湖医院中心实验室;合肥市滨湖医院干部病房;
【基金】：安徽省科技攻关项目(编号:1301042214) 安徽高校省级自然科学基金重点项目(编号:KJ2012A157) 安徽省卫生计生委中医药科研课题(编号:2016zy29)
【分类号】：R737.9

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