肝癌组织中CUL4A的表达及其对肝癌细胞生长的影响

发布时间：2018-05-27 21:22

本文选题：肝肿瘤 + CULA　；参考：《中国普通外科杂志》2017年07期

【摘要】：目的:探讨肝癌组织中泛素连接酶Cullin 4A(CUL4A)的表达情况及CUL4A对肝癌细胞生长的影响。方法:选择2013年1月—2015年12月72例肝癌组织标本及其癌旁组织标本和72例正常肝组织标本作为对照,免疫组化法检测肝癌组织、癌旁组织和正常肝组织中CUL4A的表达情况,用CUL4A-siRNA、NC-siRNA转染Hep-G2、QSG-7701肝癌细胞,CCK法检测细胞的增殖情况,PI染色、流式细胞术检测细胞周期,Transwell法检测细胞的侵袭迁移能力。结果:肝癌组织中CUL4A的表达明显高于癌旁组织和正常肝组织(t=48.657,P=0.000)。肝癌组织中CUL4A的表达和肝癌的分化程度、肿瘤直径、淋巴结转移、TNM分级有关(t=7.254、5.254、5.835、8.212,P=0.000、0.007、0.004、0.000)。转染CUL4A-siRNA的Hep-G2、QSG-7701肝癌细胞株在3 d和7 d时的OD值明显低于NC-siRNA组(t=3.132、5.264;3.152、5.834,P=0.037、0.008;0.041、0.011)。转染CUL4A-siRNA的Hep-G2、QSG-7701肝癌细胞株S期细胞比例低于NC-siRNA组(F=4.124、4.142;P=0.014、0.009)。转染CUL4A-siRNA的Hep-G2、QSG-7701肝癌细胞株侵袭转移能力低于NC-siRNA组(t=3.756、4.635;4.104、4.967,P=0.003、0.000;0.003、0.000)。结论:肝癌组织中CUL4A的表达升高,肝癌组织中CUL4A的表达与肝癌的分化程度、肿瘤直径、淋巴结转移、TNM分级有关,CUL4A能够促进肝癌细胞的增殖、细胞周期进程和侵袭迁移能力。
[Abstract]:Aim: to investigate the expression of ubiquitin ligase Cullin 4An CUL4A and the effect of CUL4A on the growth of hepatoma cells. Methods: from January 2013 to December 2015, 72 cases of HCC tissues and their adjacent tissues and 72 cases of normal liver tissues were selected as controls. The expression of CUL4A in HCC tissues, paracancerous tissues and normal liver tissues was detected by immunohistochemical method. CUL4A-siRNA-NC-siRNA transfected Hep-G2QSG-7701 hepatoma cell line was used to detect cell proliferation and Pi staining. Flow cytometry was used to detect cell cycle transwell assay. Results: the expression of CUL4A in HCC tissues was significantly higher than that in adjacent tissues and normal liver tissues. The expression of CUL4A and the differentiation degree of HCC, tumor diameter and lymph node metastasis were related to the expression of CUL4A and the classification of CUL4A in tumor tissue. The relationship between the expression of CUL4A and the degree of differentiation of HCC were related to 7.254 ~ 5.254 ~ 5.835 ~ (5.835) ~ 8.212 ~ 0.000 ~ 0.007 ~ 0.004 ~ 0.000 ~ 0.000 ~ 0. 000 ~ 0. 000 ~ 0. The OD value of Hep-G2SG-7701 hepatoma cell line transfected with CUL4A-siRNA was significantly lower than that of NC-siRNA group at 3 d and 7 d. The OD value of Hep-G2 + QSG-7701 cell line was significantly lower than that of NC-siRNA group. The percentage of S phase cells transfected with CUL4A-siRNA was lower than that of NC-siRNA group. The invasion and metastasis ability of CUL4A-siRNA transfected Hep-G2QSG-7701 hepatoma cell line was lower than that of NC-siRNA group. The ability of invasion and metastasis of Hep-G2 QSG-7701 cell line was lower than that of NC-siRNA group. Conclusion: the expression of CUL4A in HCC tissues is increased, and the expression of CUL4A in HCC tissues is related to the degree of differentiation, tumor diameter and lymph node metastasis. The expression of CUL4A can promote the proliferation, cell cycle progression and invasion and migration ability of HCC cells.
【作者单位】：齐齐哈尔医学院附属第二医院肿瘤外科;黑龙江省齐齐哈尔市第二医院外科;
【分类号】：R735.7

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