新疆多民族食管鳞癌VEGFR-2与临床病理生物学关系的研究

发布时间：2018-05-29 04:49

本文选题：血管内皮生长因子受体-2 + 食管鳞癌　；参考：《新疆医科大学》2015年硕士论文

【摘要】：目的:探讨新疆哈萨克族、维吾尔族、汉族食管鳞癌(ESCC)患者血管内皮生长因子受体-2(VEGFR-2)蛋白表达与其病理生物学关系以及其生存分析。方法:收集选取病理科收集2008-2010年食管癌组织标本170例及其对应癌旁组织标本98例,其中哈萨克族52例、维吾尔族72例、汉族46例,癌旁正常组织哈萨克族36例、维吾尔族28例、汉族34例。免疫组化采用免疫组化链霉菌抗生物素蛋白-过氧化物酶连结法(SP法)测量组织标本中VEGFR-2的蛋白表达水平。对170名患者进行随访,收集患者5年生存信息,用Kaplan-Meier法对他们的生存时间进行生存分析,Cox风险比例模型进行食管鳞癌危险因素进行分析。统计方法采用χ2检验、秩和检验(等级资料)进行。结果:170例食管鳞癌组织中VEGFR-2阳性表达率47.1%(80/170);于之相应的98例食管鳞癌患者癌旁正常组织VEGFR-2阳性表达率3.1%(3/98);二组之间VEGFR-2的阳性表达差异有统计学意义(P㩳0.01)。哈萨克族食管鳞癌组织中VEGFR-2阳表达率为46%(24/52),而40例癌旁组织中的VEGFR-2阳性表达率为5.6%(2/36),两者差异有统计学意义(P㩳0.01);维吾尔族食管鳞癌组织中VEGFR-2阳表达率为44%(32/72),而28例癌旁组织中的VEGFR-2均未见阳性表达(0/28),两者差异有统计学意义(P㩳0.01)。46例汉族食管鳞癌组织中VEGFR-2阳表达率为52%(24/46),而34例癌旁组织中的VEGFR-2阳性表达率为2.9%(1/34),两者差异有统计学意义(P㩳0.01)。食管鳞癌组织中VEGFR-2的表达与肿瘤大小、淋巴结转移、临床分期密切相关(P㩳0.05)。生存分析提示VEGFR-2阳性表达、淋巴结转移、肿瘤分期增加食管鳞癌患者的死亡风险。三民族之间VEGFR-2蛋白表达无统计学意义。结论:VEGFR-2可能成为食管鳞癌发生侵袭转移和判断预后的重要指标。
[Abstract]:Objective: to investigate the relationship between the expression of vascular endothelial growth factor receptor-2 (VEGFR-2) and its pathobiological relationship and its survival in Xinjiang Kazak Uygur and Han esophageal squamous cell carcinoma (ESCC) patients. Methods: 170 specimens of esophageal carcinoma and 98 adjacent tissues of esophageal carcinoma were collected from the Department of Pathology from 2008 to 2010, including 52 Kazakh, 72 Uygur, 46 Han, 36 Kazakh and 28 Uygur. 34 cases of Han nationality. Immunohistochemical staining was used to detect the expression of VEGFR-2 protein in tissue samples by streptomyces biotin-peroxidase (SP) method. 170 patients were followed up and 5-year survival information was collected. Survival time was analyzed by Kaplan-Meier and Cox risk ratio model was used to analyze the risk factors of esophageal squamous cell carcinoma. The statistical methods were 蠂 2 test, rank sum test (grade data). Results the positive expression rate of VEGFR-2 in 170 cases of esophageal squamous cell carcinoma was 47.1% and 80% / 170% respectively, and the positive rate of VEGFR-2 in adjacent normal tissues of 98 cases of esophageal squamous cell carcinoma was 3.1% and 3 / 98% respectively. The difference of VEGFR-2 positive expression between the two groups was statistically significant (P < 0.01). The positive expression rate of VEGFR-2 in esophageal squamous cell carcinoma of Kazak was 46 / 24 / 52%, while the positive rate of VEGFR-2 in 40 cases of adjacent tissues was 5.6 / 36, the difference was statistically significant (P < 0.01), the positive rate of VEGFR-2 positive in Uygur esophageal squamous cell carcinoma was 44 / 32 / 722, while that in 28 cases of paracancerous tissue was 44 / 722. There was no positive expression of 0 / 28% in VEGFR-2 in tissues of Han nationality. The positive expression rate of VEGFR-2 in 46 cases of esophageal squamous cell carcinoma of Han nationality was 522% / 46%, while the positive rate of VEGFR-2 expression in 34 cases of adjacent tissues was 2.9% / 34%, there was significant difference between the two groups (P 0.01). The expression of VEGFR-2 in esophageal squamous cell carcinoma was closely related to tumor size, lymph node metastasis and clinical stage. Survival analysis showed that the positive expression of VEGFR-2, lymph node metastasis and tumor stage increased the risk of death in patients with esophageal squamous cell carcinoma. There was no significant difference in the expression of VEGFR-2 protein between the three nationalities. Conclusion: VEGFR-2 may be an important indicator of invasion, metastasis and prognosis of esophageal squamous cell carcinoma.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R735.1

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