长链非编码RNA-HOTAIR对乳腺癌细胞MCF-7侵袭转移能力的影响

发布时间：2018-05-29 21:39

本文选题：长链非编码RNA + HOX转录反义RNA　；参考：《华中科技大学学报(医学版)》2017年03期

【摘要】：目的检测乳腺癌肿瘤组织中HOX转录反义RNA(HOTAIR)的表达,并观察HOTAIR对乳腺癌细胞系MCF-7侵袭转移能力的影响。方法应用荧光定量PCR检测32对乳腺癌患者的肿瘤组织和癌旁正常组织中HOTAIR的表达差异;通过慢病毒转染构建稳定高表达HOTAIR的MCF-7细胞株,Transwell检测高表达HOTAIR对细胞侵袭能力的影响,光学显微镜下观察细胞的形态学变化,Western blot法检测上皮-间质转化(EMT)相关蛋白的变化。裸鼠尾静脉注射HOTAIR高表达的MCF-7细胞,观察肝脏转移瘤的形成。结果在32对乳腺癌临床标本中,有24例肿瘤组织中HOTAIR的表达高于癌旁正常组织;MCF-7高表达HOTAIR后,细胞的侵袭能力增强,细胞发生EMT为特征的形态学变化,EMT相关的E-cadherin表达降低,而Vimentin及Slug的表达升高。高表达HOTAIR的MCF-7细胞肝脏转移瘤成瘤能力更强。结论乳腺癌临床标本中HOTAIR的表达增高,乳腺癌细胞系MCF-7中高表达HOTAIR可诱使其发生EMT,并促进其侵袭转移。
[Abstract]:Objective to detect the expression of HOX antisense RNA-HOTAIRI in breast cancer and to observe the effect of HOTAIR on the invasion and metastasis of breast cancer cell line MCF-7. Methods fluorescent quantitative PCR was used to detect the difference of HOTAIR expression in tumor tissues and adjacent normal tissues of breast cancer patients, and the effect of high expression HOTAIR on the invasion of breast cancer cells was detected by lentivirus transfection to construct MCF-7 cell line with stable and high expression of HOTAIR. The morphological changes of cells were observed under optical microscope. The changes of EMT-related proteins were detected by Western blot. MCF-7 cells with high expression of HOTAIR were injected into tail vein of nude mice to observe the formation of liver metastases. Results in 32 clinical specimens of breast cancer, the expression of HOTAIR in 24 cases of breast cancer tissues was higher than that of the adjacent normal tissues with high expression of HOTAIR, and the invasive ability of the cells was enhanced, and the morphological changes characterized by the occurrence of EMT in the cells decreased the expression of E-cadherin associated with EMT. However, the expression of Vimentin and Slug increased. MCF-7 cells with high expression of HOTAIR have stronger ability of liver metastasis. Conclusion the expression of HOTAIR is increased in clinical samples of breast cancer, and the overexpression of HOTAIR in breast cancer cell line MCF-7 can induce the development of HOTAIR and promote its invasion and metastasis.
【作者单位】：河南省人民医院乳腺外科;
【基金】：河南省科技厅基金资助项目(No.152300410153)
【分类号】：R737.9

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