低频脉冲电刺激对黑色素瘤小鼠的疗效及对其心肌损伤的研究

发布时间：2018-05-30 19:21

本文选题：脉冲电刺激 + 黑色素瘤　；参考：《重庆医科大学》2015年硕士论文

【摘要】：目的：观察不同参数的低频脉冲电刺激对B16-F10黑色素瘤小鼠的抑瘤效应,评估其对心脏结构及功能的影响。方法：将由B16-F10细胞接种产生的荷瘤BALB/c小鼠随机分为4组。治疗组选择在场强20V/cm、脉宽lms,频率分别为lOHz (10Hz组)、20Hz (20Hz组)和25Hz (25Hz组)的脉冲电刺激30min/d作用于荷瘤小鼠,以不用电刺激为对照组(每组10只),治疗时监测各小鼠心电图变化情况。用此参数脉冲电刺激作用于荷瘤小鼠7d后测量比较各组肿瘤体积及生存期的变化,HE染色观察肿瘤及心脏组织形态学变化,免疫组化法比较各组小鼠肿瘤组织中S-100B的变化。ELISA法检测小鼠血清肌钙蛋白T(cTnT)及肌酸激酶同工酶(CK-MB)的变化。结果：低频脉冲电刺激治疗15d后10Hz组、20Hz组和25Hz组的肿瘤体积分别为463.026±33.03、248.609±34.620和229.879±15.860mm3,显著小于对照组(2026.989±133.358mm3,P0.05)。且20Hz组生存率延长率(67.40%)明显高于10Hz组(15.47%)和25Hz组(8.8%)；随着频率的增加,S-100B蛋白阳性表达依次减少；25Hz组小鼠心律失常评分、血清cTnT、CK-MB显著高于对照组(P0.05)；与对照组相比,10Hz与20Hz组小鼠心律失常评分、cTnT、CK-MB差异无统计学意义(P0.05)。结论：低频脉冲电刺激对荷瘤小鼠具有抑瘤作用,在20V/cm、20Hz时抑制作用最强,且不影响小鼠正常的心电活动。
[Abstract]:Aim: to observe the inhibitory effect of low frequency pulse electric stimulation on B16-F10 melanoma mice and evaluate its effect on cardiac structure and function. Methods: BALB/c mice inoculated with B16-F10 cells were randomly divided into 4 groups. The treatment group was treated with pulsed electric stimulation (30min/d) at the field intensity of 20V / cm, pulse width of lms, frequency of lOHz 10Hz group (20Hz / 20Hz) and 25Hz group of 25Hz). The control group (10 rats in each group) was treated with no electrical stimulation. The electrocardiogram changes of each mouse were monitored during the treatment. The changes of tumor volume and survival time in each group were measured and compared after 7 days of pulsed electrical stimulation with this parameter. The morphological changes of tumor and heart were observed by HE staining. The changes of S-100B in tumor tissue of each group were compared by immunohistochemical method. The changes of serum troponin TnTnT and creatine kinase isoenzyme (CK-MBB) were detected by Elisa. Results: the tumor volume of 10Hz group was 463.026 卤33.03248.609 卤34.620 and 229.879 卤15.8603 mm respectively, which was significantly lower than that of control group (2026.989 卤133.358mm P0.05). The survival rate of 20Hz group was significantly higher than that of 10Hz group (67.40%) and 25Hz group (8.8%), and the positive expression of S-100B protein decreased in turn with the increase of frequency. The serum cTnTnTnTnCK-MB was significantly higher than that of the control group (P0.05), and the arrhythmia score of 10 Hz and 20Hz group was not significantly different from that of the control group (P 0.05). Conclusion: low frequency pulse electric stimulation has inhibitory effect on tumor-bearing mice, and it has the strongest inhibitory effect at 20 V / cm ~ (-1) 20 Hz, and does not affect normal ECG activity of mice.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R739.5

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