TFF2表达及幽门螺杆菌感染在胃癌患者中的临床意义

发布时间：2018-05-30 21:32

  本文选题：胃癌 + TFF2　； 参考：《川北医学院》2017年硕士论文

【摘要】：目的:检测胃癌组织中TFF2蛋白的表达及患者幽门螺杆菌感染情况,研究TFF2蛋白表达、幽门螺杆菌感染与胃癌患者临床病理特征之间的关系,探讨TFF2蛋白表达及幽门螺杆菌感染在胃癌患者中的临床意义。方法:收集四川省人民医院2010年12月-2012年1月入院确诊并首次行胃癌手术患者82例作为研究对象。应用免疫组化方法检测胃癌组织及正常胃组织中TFF2蛋白表达情况,应用免疫组化方法检测胃癌患者幽门螺旋杆菌的感染情况,分析TFF2蛋白表达、幽门螺杆菌感染与胃癌患者临床病理之间的关系。并对患者进行临床病理资料及随访资料分析,采用Kaplan-Meier方法计算患者生存率,Log-Rank检验进行单因素分析,Cox比例风险回归进行多因素分析TFF2表达、幽门螺杆菌感染与胃癌患者预后的关系。结果:1.胃癌患者临床病理特征病例总数82例。其中男55例,女27例,男女比例为2.0:1。年龄37-82岁,平均年龄59.2±11.5岁。临床TNM分期:I期14例,II期19例,III期42例,IV期7例。肿瘤浸润深度方面,以T3最多,其中T1期10例,T2期8例,T3期60例,T4期4例。无淋巴结转移32例,有淋巴结转移50例。胃癌病理分化程度:中低分化55例,高分化27例。肿瘤大小:≤4cm 47例,4cm 35例。2.TFF2蛋白表达、HP感染与临床病理之间的关系TFF2蛋白主要表达在胞质上。在30例正常胃组织标本中TFF2阴性率和阳性率百分比为16.7%(5/30)、83.3%(25/30)。在82例患者胃癌组织标本中TFF2表达阴性率和阳性率百分比分别为45.1%(37/82)、54.9%(45/82)。二者比较,胃癌组织中TFF2表达比正常胃组织降低,差异有统计学意义(P值0.05)。将胃癌患者各临床特征与TFF2表达的相关性进行分析,发现TFF2的表达与组织分化程度有关,差异有统计学意义(P值0.05)。在82例胃癌患者中,HP感染阳性病例50例,HP阴性病例32例,HP感染阳性率为61.0%。将胃癌患者各临床特征与HP感染相关性进行分析,发现HP的感染与患者TNM分期和浸润深度有关,差异有统计学意义(P值0.05)。3.预后分析单因素分析显示年龄、TNM分期、淋巴结转移、浸润深度、分化程度、肿瘤大小及TFF2蛋白表达、HP感染是影响胃癌患者生存的相关因素。进一步Cox比例风险模型多因素分析显示淋巴结转移、肿瘤大小、TFF2表达、幽门螺杆菌感染是影响胃癌患者预后的独立因素。结论:1.TFF2在胃癌组织中的表达比正常胃组织中明显降低。TFF2的表达与肿瘤分化程度有关。幽门螺杆菌感染与肿瘤浸润深度、TNM分期有关。2.TFF2的表达、幽门螺杆菌感染可以作为胃癌患者预后的独立因素,检测胃癌组织TFF2蛋白的表达、患者幽门螺杆菌感染情况可能对判断患者预后有帮助。3.TFF2在胃癌组织中的表达与患者幽门螺杆菌感染之间呈负相关,提示二者之间可能存在某种相互作用机制影响胃癌的发生发展。
[Abstract]:Objective: to detect the expression of TFF2 protein in gastric cancer and the infection of Helicobacter pylori, and to study the relationship between the expression of TFF2 protein, the relationship between Helicobacter pylori infection and the clinicopathological features of gastric cancer. To investigate the expression of TFF2 protein and the clinical significance of Helicobacter pylori infection in patients with gastric cancer. Methods: from December 2010 to January 2012, 82 patients with gastric cancer were enrolled in Sichuan Provincial people's Hospital. Immunohistochemical method was used to detect the expression of TFF2 protein in gastric cancer tissues and normal gastric tissues. The infection of Helicobacter pylori in gastric cancer patients was detected by immunohistochemical method, and the expression of TFF2 protein was analyzed. Relationship between Helicobacter pylori infection and clinicopathology in patients with gastric cancer. The clinicopathological data and follow-up data of the patients were analyzed. The survival rate was calculated by Kaplan-Meier method. The expression of TFF2 was analyzed by univariate analysis and Cox proportional risk regression. Relationship between Helicobacter pylori infection and prognosis in patients with gastric cancer. The result is 1: 1. There were 82 cases of gastric cancer with clinicopathological features. There were 55 males and 27 females, with a ratio of 2.0: 1. The average age was 59.2 卤11.5 years. There were 14 cases of stage II and 19 cases of stage III in TNM stage I. There were 42 cases with stage IV and 7 cases with stage IV. In terms of depth of tumor invasion, T3 was the most frequent, of which 10 cases were in T1 stage and 8 cases in T _ 2 stage, 60 cases were in T _ 3 stage and 4 cases were in T _ 4 stage. There were 32 cases without lymph node metastasis and 50 cases with lymph node metastasis. The pathological differentiation degree of gastric cancer: 55 cases with middle and low differentiation and 27 cases with high differentiation. Tumor size: 鈮，

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