精氨酸酶抑制剂nor-NOHA诱导HepG2肝癌细胞凋亡并抑制其侵袭和迁移

发布时间：2018-05-31 23:30

本文选题：肝细胞癌 + 精氨酸酶　；参考：《细胞与分子免疫学杂志》2017年04期

【摘要】：目的探讨精氨酸酶抑制剂nor-NOHA对HepG2肝癌细胞的抑制作用及其机制。方法采用CCK-8法及流式细胞术检测(0.0、0.5、1.0、2.0、3.0)ng/μL nor-NOHA对人肝癌HepG2细胞的增殖抑制作用和细胞凋亡的促进作用;Western blot法检测其对细胞内精氨酸酶1(Arg1)、P53、基质金属蛋白酶2(MMP-2)以及上皮钙黏素(ECD)蛋白表达的影响;实时定量PCR检测诱导型一氧化氮合酶(i NOS)m RNA的表达;Griess法检测各组HepG2细胞产生的一氧化氮(NO)浓度;TranswellTM实验及细胞划痕实验测定HepG2细胞的侵袭、迁移能力。结果 nor-NOHA抑制肝癌细胞的增殖并诱导其凋亡;nor-NOHA降低Arg1、MMP-2的表达水平,升高P53、ECD的水平并增加NO的合成;nor-NOHA降低肝癌细胞的侵袭、迁移能力。结论nor-NOHA通过抑制Arg1,可诱导肝癌细胞凋亡并降低其侵袭、迁移能力,其机制与升高i NOS的表达,产生高浓度NO有关。
[Abstract]:Objective to investigate the inhibitory effect of arginase inhibitor nor-NOHA on HepG2 hepatoma cells and its mechanism. Methods CCK-8 assay and flow cytometry were used to detect the inhibitory effect of nor-NOHA on proliferation and apoptosis of human hepatoma HepG2 cells. Western blot assay was used to detect the intracellular arginase 1 Arg1 P53, matrix metalloproteinase 2 (MMP-2) and epithelial cadherin (E-cadherin) in human hepatoma HepG2 cells. The effect of ECD) protein expression; The expression of inducible nitric oxide synthase (iNOS) NOS)m RNA was detected by real-time quantitative PCR. The concentration of nitric oxide (no) produced by HepG2 cells in each group was detected by Griess method. The invasion and migration ability of HepG2 cells was measured by cell scratch assay and cell scratch assay. Results nor-NOHA inhibited the proliferation of hepatoma cells and induced apoptosis. Nor-NOHA decreased the expression of Arg1 and MMP-2, increased the level of P53, and increased the synthesis of no. Nor-NOHA decreased the invasion and migration ability of hepatoma cells. Conclusion nor-NOHA can induce apoptosis and decrease the invasion and migration ability of hepatoma cells by inhibiting Arg 1. The mechanism is related to the increase of I NOS expression and the production of high concentration no.
【作者单位】：重庆医科大学附属第一医院肝胆外科;
【基金】：国家自然科学基金(81372481)
【分类号】：R735.7

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