Smad1在胃腺癌组织中的表达及对胃腺癌细胞迁移能力的影响研究

发布时间：2018-06-01 06:37

本文选题：胃腺癌 + Akt信号通路　；参考：《中国免疫学杂志》2017年06期

【摘要】：目的:研究Smad1在胃腺癌组织中的表达及对胃腺癌细胞迁移能力的影响。方法:提取收集的胃腺癌组织及对应的癌旁组织中的蛋白,Western blot检测Smad1的表达水平。以胃腺癌细胞HGC-27为研究对象,细胞转染过表达Smad1的载体(p-EGFP-C1/Smad1)和Smad1小干扰RNA(Smad1 siRNA),同时转染p-EGFP-C1和siRNA control为对照。细胞划痕实验检测细胞迁移能力。Western blot检测细胞中MMP-9、MMP-2、p-Akt、Akt的表达水平。Akt信号通路抑制剂LY294002(20μg/ml)作用于胃腺癌细胞HGC-27,MTT检测细胞增殖情况,细胞划痕实验检测细胞迁移能力。Western blot检测MMP-9、MMP-2、p-Akt、Akt蛋白表达水平。结果:胃腺癌组织中Smad1的水平明显低于癌旁组织(P0.01)。p-EGFP-C1/Smad1组细胞存活率和迁移率均明显低于p-EGFP-C1组(P0.01)。Smad1 siRNA组细胞存活率和迁移率均明显高于siRNA control组(P0.01)。p-EGFP-C1、p-EGFP-C1/Smad1、siRNA control、Smad1 siRNA组细胞中Akt蛋白表达水平没有变化。p-EGFP-C1/Smad1组细胞MMP-9、MMP-2、p-Akt蛋白表达水平明显低于p-EGFP-C1组(P0.01)。Smad1 siRNA组细胞MMP-9、MMP-2、p-Akt蛋白表达水平明显高于siRNA control(P0.01)。胃腺癌细胞与Akt信号通路抑制剂作用后细胞增殖和迁移趋势与p-EGFP-C1/Smad1组一致。结论:Smad1在胃腺癌组织中低表达。Smad1能够抑制胃腺癌细胞增殖和迁移,作用机制与Akt信号通路有关。
[Abstract]:Aim: to study the expression of Smad1 in gastric adenocarcinoma tissues and its effect on the migration ability of gastric adenocarcinoma cells. Methods: Western blot was used to detect the expression of Smad1 in gastric adenocarcinoma tissues and adjacent tissues. Gastric adenocarcinoma cells (HGC-27) were transfected with pEGFP-C1 / Smad1) and Smad1 small interfering RNA(Smad1 siRNAs, and p-EGFP-C1 and siRNA control were transfected as controls. The effect of LY294002(20 渭 g / ml, an Akt signaling pathway inhibitor, on the proliferation of gastric adenocarcinoma cells was detected by HGC-27MTT assay. Western blot was used to detect the expression level of MMP-9, MMP-2MMP, p-AktAMT, and LY294002(20 渭 g / ml, an inhibitor of Akt signaling pathway, to detect the proliferation of gastric adenocarcinoma cells. The cell migration ability was detected by cell scratch assay. The expression of MMP-9 and MMP-2p-Akt protein was detected by Western blot. Results: the level of Smad1 in gastric adenocarcinoma tissues was significantly lower than that in the adjacent tissues (P 0.01). P-EGFP-C 1 / Smad1 group. The cell survival rate and migration rate were significantly lower than those in the p-EGFP-C1 group P0.01 Smad1 siRNA group. P-EGFP-C1p-EGFP-C1% Smad1 siRNA control Smad1 siRNA group was significantly higher than the siRNA control group in cell viability and mobility. The expression level of MMP-9, MMP-2mp-Akt protein in the pEGFP-C1 / Smad1 group was significantly lower than that in the p-EGFP-C1 group (P0.01and Smad1 siRNA group), and the expression level of MMP-9MP-Akt protein was significantly higher than that in the siRNA control group (P0.01). The proliferation and migration of gastric adenocarcinoma cells treated with Akt signaling pathway inhibitor were consistent with those of p-EGFP-C1/Smad1 group. Conclusion the low expression of Smad1 in gastric adenocarcinoma can inhibit the proliferation and migration of gastric adenocarcinoma cells, and the mechanism is related to the Akt signaling pathway.
【作者单位】：河北医科大学第一医院病理科;河北医科大学第一医院骨科;
【基金】：河北省卫计委青年科技课题(20150642)项目
【分类号】：R735.2

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