细胞外基底刚度调控宫颈癌细胞迁移的microRNA的筛选

发布时间：2018-06-01 10:50

本文选题：宫颈癌 + microRNA芯片　；参考：《中国微创外科杂志》2017年07期

【摘要】：目的通过microRNA芯片筛选不同基底刚度影响宫颈癌细胞迁移过程中的关键microRNA的候选者。方法用丙烯酰胺及双丙烯酰胺的不同比例配置基底刚度为1、20 k Pa的人工基底膜,在其上种植宫颈癌细胞系Siha,36 h后收取细胞提取总RNA,用microRNA芯片及qRT-PCR方法筛选2种基底刚度条件下的差异表达microRNA。结果在2种不同刚度条件下生长的宫颈癌细胞系Siha共有173个表达差异的microRNA,其中包括70个表达上调的microRNA和103个表达下调的microRNA。在宫颈癌细胞系Siha、Hela、Caski中,检测与宫颈癌细胞迁移相关的miR-21、miR-125a、miR-75b、miR-150、miR-595、miR-218、miR-200b、miR-107、miR-183等表达与microRNA芯片结果一致。结论不同基底刚度可引起宫颈癌细胞系Siha的microRNA表达谱不同,microRNA在基底刚度调控宫颈癌细胞迁移的过程中可能具有重要作用。
[Abstract]:Objective to select the candidate of the key microRNA in the migration process of cervical cancer cells by microRNA chip. Methods the artificial basal membrane with the basal stiffness of 1,20 K Pa was arranged in different proportion of acrylamide and double acrylamide, and the cervical cancer cell line Siha was planted on it, and the total RNA was collected after 36 h, and microRN was used in microRN. A chip and qRT-PCR method screened the differential expression of microRNA. under 2 basal stiffness conditions. There were 173 differentially expressed microRNA in the cervical cancer cell line Siha grown under 2 different stiffness conditions, including 70 up regulated microRNA and 103 down regulated microRNA. in the cervical cancer cell line Siha, Hela, Caski. The expression of miR-21, miR-125a, miR-75b, miR-150, miR-595, miR-218, miR-200b, miR-107, miR-183, which is related to the migration of cervical cancer cells, is in agreement with the microRNA chip results. Conclusion different basal stiffness can cause the Siha microRNA expression profiles of cervical cancer cell lines, which may be used in the process of regulating cervical cancer cell migration by basal stiffness. It has an important role.
【作者单位】：北京大学第三医院妇产科;北京大学第三医院心内科;北京大学第三医院血管医学研究所;
【基金】：国家自然科学基金(项目编号:81472429,81471893,81270157) 973课题计划(项目编号:2013CB933702,2014CBA02003)
【分类号】：R737.33

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