五个与肾透明细胞癌患者预后显著相关lncRNA的发现

发布时间：2018-06-02 10:25

本文选题：lncRNAs + 肾透明细胞癌　；参考：《华东师范大学》2017年硕士论文

【摘要】：癌症是一种形成原因特别复杂的慢性疾病,遗传方面的因素、生活环境的好坏都可能会决定个体是否会患上癌症。近期的一些研究报道表明,lncRNAs(long non-coding RNAs,长链非编码核糖核酸)在肿瘤发生过程和癌症患者的预后中扮演重要的角色,由此激发了人们对lncRNAs在癌症预后中具有潜在临床相关的作用及机理进行了一系列研究。肾细胞癌(简称肾癌)是人体泌尿系统器官中恶性度较高的一种肿瘤,也是人体所有肿瘤中最常见的肿瘤之一,起源于肾实质泌尿小管上皮系统的恶性肿瘤,又被称为肾腺癌,占所有肾恶性肿瘤的85%～95%。通常病理学家将肾细胞癌分为4种类型:肾透明细胞癌、乳头状肾细胞癌、嫌色细胞性肾细胞癌以及Bellini集合管癌。其中,绝大多数为肾透明细胞癌,占肾癌的70%～80%,其癌细胞常排列成片状、条索状、腺泡状或管状。然而,lncRNAs标记作为一种生物标记在肾透明细胞癌(ccRCC,clear cell renal cell carcinoma)患者的预后中起什么样作用,尚未被深入研究和讨论。因此,我们试图去寻找与肾透明细胞癌患者预后显著相关的一些lncRNAs。首先,我们在癌症和肿瘤基因图谱计划公共数据库(TCGA)中,提取了带有临床信息数据的440个肾透明细胞癌患者数据,并对这组数据中lncRNAs的表达值进行分析。在对总的数据集分组后的训练集以及测试集中,我们发现并验证了五个与肾透明细胞癌患者预后显著相关 lncRNAs(AC069513.4、AC003092.1、CTC-205M6.2、RP11-507K2:3、U91328.21)。基于这五个预后相关的lncRNAs,样本中的肾透明细胞癌患者可被分为高风险和低风险组两种组别。其中,存在于不同组别的患者的存活率明显不同。多变量Cox回归分析表明,这五个预后相关的lncRNAs具有相对于其他临床因素而言的独立的预后价值。进一步的功能富集分析还显示出这五个预后相关的lncRNAs在肿瘤形成过程中所发挥的潜在作用。所有的生存分析的结果都表明,这五个预后相关的lncRNAs对于肾透明细胞癌患者预后来说,很有可能作为一个独立而又重要的生存指标。
[Abstract]:Cancer is a very complicated chronic disease, genetic factors, living conditions can determine whether an individual will have cancer. Recent studies have shown that long non-coding RNASs play an important role in tumorigenesis and prognosis in cancer patients. This has stimulated a series of studies on the potential clinicopathological roles and mechanisms of lncRNAs in cancer prognosis. Renal cell carcinoma (RCC) is a kind of tumor with high malignancy in the organs of human urinary system. It is also one of the most common tumors in all human tumors. It originates from the malignant tumor of renal parenchymal urothelial system and is also called renal adenocarcinoma. It accounts for 85% of all malignant renal tumors. Usually, renal cell carcinoma is classified into four types by pathologists: clear cell carcinoma of kidney, papillary renal cell carcinoma, chromophobic renal cell carcinoma and Bellini collecting tube carcinoma. Most of them are clear cell carcinoma of the kidney, accounting for 70% of renal carcinoma. Its cancer cells are usually arranged in sheet, stripe, acinar or tubular form. However, the role of lncRNAs as a biomarker in the prognosis of patients with clear renal cell carcinoma (CCCC) clear cell renal cell carcinoma) has not been further studied and discussed. Therefore, we try to look for some lncRNAs that are significantly associated with the prognosis of renal clear cell carcinoma. Firstly, we extracted 440 data of renal clear cell carcinoma with clinical data from cancer and tumor gene map program public database (TCGA), and analyzed the expression of lncRNAs in these data. In the training set and test set after grouping the total data sets, we found and verified five significant correlations between the prognosis of clear cell renal carcinoma patients and the prognosis of five patients with clear cell carcinoma of kidney AC069513.4% AC003092.1 CTC-205M6.2n RP11-507K2: 3 U91328.21. Based on the five prognostic factors lncRNAs. the patients with renal clear cell carcinoma in the sample were divided into two groups: high risk group and low risk group. The survival rates of patients in different groups were significantly different. Multivariate Cox regression analysis showed that these five prognostic lncRNAs had independent prognostic value compared with other clinical factors. Further functional enrichment analysis also revealed the potential role of these five prognostic lncRNAs in tumor formation. All the results of survival analysis show that these five prognostic lncRNAs are likely to be an independent and important prognostic marker for the prognosis of renal clear cell carcinoma (RCC) patients.
【学位授予单位】：华东师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.11

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