胃癌长链非编码RNA SNHG16异常表达及其临床病理意义

发布时间：2018-06-03 12:29

  本文选题：胃癌 + 长链非编码RNA　； 参考：《东南大学学报(医学版)》2017年04期

【摘要】：目的:检测长链非编码RNA SNHG16在胃癌组织与癌旁组织中的表达及其临床意义。方法:实时荧光定量PCR(quantitative real time-PCR,qRT-PCR)法检测SNHG16在41例胃癌组织及5株不同分化程度细胞系和胃黏膜上皮细胞中的表达情况,分析SNHG16表达与胃癌临床病理的相关性。结果:胃癌组织中SNHG16相对于癌旁组织其表达量为(2.64±1.59),表达显著上调且与胃癌侵袭相关(P0.05,r=0.361)。与GES-1细胞株比较,HGC-27中SNHG16相对表达量为(4.21±0.69),MGC-803为(1.99±0.21),BGC-803为(1.90±0.44),SGC-7901为(0.76±0.05),AGS为(3.36±0.81);SNHG16在SGC-7901中低表达,但差异无统计学意义(P0.05),在其他4株细胞中显著高表达(P0.01)。结论:SNHG16在胃癌组织中高表达,且其表达水平与胃癌侵袭程度相关,可成为胃癌预后监测及治疗的靶点。
[Abstract]:Aim: to detect the expression and clinical significance of long-chain non-coding RNA SNHG16 in gastric cancer and paracancerous tissues. Methods: the expression of SNHG16 in 41 cases of gastric carcinoma, 5 different differentiation cell lines and gastric epithelial cells was detected by real-time fluorescence quantitative PCR(quantitative real time-PCRQRT-PCR. the correlation between SNHG16 expression and clinicopathology of gastric cancer was analyzed. Results: the expression of SNHG16 in gastric cancer tissues was 2.64 卤1.59, which was significantly up-regulated and correlated with the invasion of gastric carcinoma. Compared with GES-1 cell line, the relative expression of SNHG16 in HGC-27 was 4.21 卤0.69 MGC-803, 1.99 卤0.21, BGC-803, 1.90 卤0.44, SGC-7901, 0.76 卤0.05AGS, 3.36 卤0.81HG16 in SGC-7901, but the difference was not statistically significant (P 0.05). Conclusion the high expression of the protein SNHG16 is associated with the degree of invasion of gastric cancer, which may be a target for the prognosis monitoring and treatment of gastric cancer.
【作者单位】： 贵州医科大学医学检验学院临床生化教研室;贵州医科大学附属医院生化科;贵州医科大学附属医院胃肠外科;
【基金】：国家自然科学基金资助项目(81460364)
【分类号】：R735.2
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本文编号：1972784