胃癌患者血清中食欲素A的水平及其受体表达的研究

发布时间：2018-06-03 16:14

  本文选题：胃癌 + 食欲素A　； 参考：《宁夏医科大学》2017年硕士论文

【摘要】：目的通过酶联免疫吸附试验(ELISA)及免疫组织化学染色法检测胃癌患者血清中食欲素A(OXA)的水平及组织中食欲素1型受体(OX1R)的表达情况,探讨OXA在胃癌发生、发展过程中的作用。方法1.通过酶联免疫吸附试验(ELISA)检测50例胃癌患者血清和30例健康体检者血清中食欲素A的含量,并进行统计学分析;2.通过免疫组织化学染色法检测胃癌组织与正常胃黏膜组织中食欲素受体1的分布情况,并进行统计学分析。结果(1)胃癌患者血清OXA水平明显高于健康体检者,差异(P0.01)存在显著性统计学意义;(2)将两组血清标本按年龄各分为两组(≤65岁、65岁)。两组血清标本中“≤65岁”组的OXA水平均高于“65岁”组,差异(t=0.59、t=0.68,P0.05)无统计学意义;(3)将胃癌血清标本按其分化程度将其分为未分化胃癌、低分化胃癌、中分化胃癌、高分化胃癌组,OXA水平在胃癌分化程度上(F=0.05,P0.05)无明显统计学差异。(4)正常胃粘膜组织标本中食欲素1型受体(OX1R)阳性表达率60%,平均染色分值(3.67±1.80);胃癌组织OX1R阳性表达率94.7%,平均染色分值(7.53±3.20),与正常组织相比,胃癌组织中OX1R阳性表达率明显增高(P0.01),且阳性平均染色分值亦显著高于正常组(P0.01);(5)将胃癌组织标本按照胃癌分化程度,分为分化型胃癌及未分化型胃癌两组,分化型胃癌OX1R阳性表达的平均染色分值为(6.86±2.87),未分化型胃癌阳性表达的平均染色分值为(9.88±2.41),与分化型胃癌相比,未分化胃癌组织染色分值显著增高(P0.05),且所检测到的OX1R主要位于癌细胞胞浆和胞膜上,呈浅黄色至棕黄色颗粒。结论胃癌患者血清中食欲素A水平增高,胃癌组织中OX1R表达增多,提示食欲素系统在胃癌的发生、发展中很可能起着促进作用。
[Abstract]:Objective to investigate the role of OXA in the carcinogenesis and development of gastric cancer by using Elisa and immunohistochemical staining to detect the level of orexin A OXA in serum and the expression of orexin type 1 receptor (OX1R) in the tissues of patients with gastric cancer. Method 1. The levels of orexin A in serum of 50 patients with gastric cancer and 30 healthy controls were detected by Elisa. The distribution of orexin receptor 1 in gastric cancer and normal gastric mucosa was detected by immunohistochemical staining. Results (1) the serum OXA level in patients with gastric cancer was significantly higher than that in healthy controls (P 0.01). The serum samples were divided into two groups according to their age (鈮，

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