地西他滨抑制A431细胞生物学功能的机制

发布时间：2018-06-05 18:13

本文选题：地西他滨 + A　；参考：《中国医科大学学报》2017年12期

【摘要】：目的检测地西他滨对A431细胞生物学功能的影响并初步探讨其机制。方法将0、5、10和20μmol/L的地西他滨分别作用于A431细胞,通过MTT法观察地西他滨对细胞增殖的影响,通过Western blotting和实时PCR检测地西他滨作用于A431细胞后细胞增殖相关蛋白Cyclin B1、PCNA和CDC2的改变。通过Hoechst 33258染色检测0、5和10μmol/L地西他滨对A431细胞凋亡状态的影响,采用Western blotting和实时PCR检测地西他滨对A431细胞凋亡相关蛋白Bax和Bcl-2的影响。采用transwell实验检测细胞侵袭转移情况,并通过Western blotting和实时PCR检测0、5和10μmol/L的地西他滨对侵袭转移相关蛋白MMP2的影响。结果 MTT检测发现,5、10和20μmol/L的地西他滨作用于A431细胞24 h后对细胞增殖的抑制率分别为(43.81±1.53)%、(48.64±4.65)%和(50.69±4.99)%,随着药物的浓度增加,对A431细胞的增殖抑制率显著增加(P0.05)。Hoechst 33258染色发现,地西他滨可以显著促进细胞凋亡,transwell实验也证明地西他滨可以明显抑制A431细胞的转移能力。Western blotting和实时PCR结果显示,地西他滨影响A431细胞中Cyclin B1、CDC2、PCNA、Bax、Bcl-2和MMP2蛋白及m RNA水平,地西他滨处理组与对照组比较,差异均有统计学意义(P0.05)。结论地西他滨对外阴鳞状细胞癌的生物学功能有很强的抑制作用,可以作为治疗外阴鳞状细胞癌的化疗药物。
[Abstract]:Objective to investigate the effect of dietabine on biological function of A 431 cells and its mechanism. Methods the cell proliferation of A431 cells was observed by MTT, and the changes of Cyclin B1P and CDC2 were detected by Western blotting and real-time PCR. The apoptosis of A431 cells was detected by Hoechst 33258 staining, and the apoptosis related proteins Bax and Bcl-2 of A431 cells were detected by Western blotting and real-time PCR. Transwell assay was used to detect the invasion and metastasis of the cells, and the effects of 0 渭 mol/L and 10 渭 mol/L on the invasion and metastasis associated protein MMP2 were detected by Western blotting and real-time PCR. Results the inhibitory rates of dietabine on A431 cell proliferation were 43.81 卤1.53 卤4.65% and 50.69 卤4.99%, respectively. With the increase of drug concentration, the inhibition rate of proliferation of A431 cells was significantly increased with the increase of drug concentration. Hoechst 33258 staining showed that the inhibitory rate of dietabine on A431 cell proliferation was significantly higher than that of control group. Dietabine could significantly promote apoptosis. The transwell assay also demonstrated that dietabine could significantly inhibit the metastatic ability of A431 cells. Western blotting and real-time PCR results showed that dietabine affected the expression of Bcl-2, MMP2 and m RNA in A431 cells. Compared with the control group, the difference between the two groups was statistically significant (P 0.05). Conclusion Detabine has a strong inhibitory effect on the biological function of vulvar squamous cell carcinoma and can be used as a chemotherapeutic drug for vulvar squamous cell carcinoma.
【作者单位】：中国医科大学附属盛京医院妇产科;沈阳医学院附属第二医院普外科;
【基金】：沈阳市科学技术计划(F15-199-1-34)
【分类号】：R737.35

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