SAHA对胃癌MGC-803细胞增殖与周期及凋亡的影响

发布时间：2018-06-06 01:03

本文选题：组蛋白去乙酰化酶抑制剂 + 胃癌　；参考：《西安交通大学学报(医学版)》2017年01期

【摘要】：目的研究组蛋白去乙酰化酶抑制剂(SAHA)对胃癌细胞株MGC-803增殖、凋亡及周期的影响。方法 MTT比色法观察不同药物浓度SAHA作用于培养胃癌细胞株MGC-803时的增殖变化,初步选取药物作用48h时的合适药物浓度,进一步用流式细胞术检测1、2μmol/L SAHA对MGC-803细胞凋亡及周期的影响,Western blot检测乙酰化组蛋白3、4及cyclin D1蛋白的表达情况。结果与正常对照组相比,SAHA处理组细胞增殖能力降低,凋亡率升高,细胞周期内G1期比率升高,差异均具有统计学意义(P0.05);Western blot检测结果显示,与正常对照组相比,SAHA处理组的乙酰化组蛋白3、乙酰化组蛋白4升高,而cyclin D1含量降低,差异均具有统计学意义(P0.01)。结论组蛋白去乙酰化酶抑制剂SAHA可以抑制MGC-803细胞增殖,通过G1-S期阻滞抑制细胞周期,促进细胞凋亡,可能与促进乙酰化组蛋白3及乙酰化组蛋白4的表达增多有关。
[Abstract]:Objective to study the effects of protein deacetylase inhibitor (PNA) on proliferation, apoptosis and cell cycle of gastric cancer cell line MGC-803. Methods MTT colorimetric assay was used to observe the proliferation of gastric cancer cell line MGC-803 treated with different concentrations of SAHA. The effect of 1 渭 mol/L SAHA on the apoptosis and cell cycle of MGC-803 cells was further detected by flow cytometry. The expression of acetylated histone 3n4 and cyclin D1 protein was detected by Western blot. Results compared with the control group, the proliferation ability, apoptosis rate and G1 phase ratio in the SAHA treated group were significantly lower than those in the normal control group, and the difference was statistically significant (P 0.05). Compared with the control group, the acetylated histone 3 and acetylated histone 4 increased, while the content of cyclin D1 decreased in SAHA group, the difference was statistically significant (P 0.01). Conclusion histone deacetylase inhibitor SAHA can inhibit the proliferation of MGC-803 cells, inhibit cell cycle and promote cell apoptosis through G1-S phase arrest, which may be related to the increase of acetylated histone 3 and acetylated histone 4 expression.
【作者单位】：锦州医科大学;锦州医科大学附属第一医院;
【分类号】：R735.2

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