冬凌草活性成分KY3对食管癌EC109细胞增殖的抑制作用

发布时间：2018-06-06 02:35

本文选题：EC细胞 + 凋亡　；参考：《郑州大学学报(医学版)》2017年01期

【摘要】：目的:探讨冬凌草活性成分KY3对食管癌细胞EC109增殖的抑制作用及其机制。方法:MTT法测定0、10、20、30、40、60、80、100μmol/L KY3对EC109细胞增殖的影响;流式细胞术检测0、10、20、30μmol/L KY3对EC109细胞凋亡和细胞周期分布的影响,免疫荧光法观察KY3对EC109细胞中微管结构的影响,Hoechst 33258染色观察KY3作用后EC109细胞核的形态。结果:KY3能够抑制EC109细胞的体外增殖;KY3处理后EC109细胞周期阻滞在G2/M期并发生凋亡(P0.05);KY3作用于EC109细胞后,微管遭到破坏,随着KY3剂量的增加,α-微管蛋白逐渐发生降解并出现细胞核固缩、浓染等典型的凋亡形态。结论:冬凌草活性成分KY3在体外对EC109细胞具有增殖抑制作用,其机制可能与破坏细胞微管结构、G2/M期细胞周期阻滞及诱导细胞凋亡有关。
[Abstract]:Aim: to investigate the inhibitory effect of KY3 on the proliferation of esophageal cancer cell line EC109 and its mechanism. Methods the cell proliferation of EC109 cells was determined by cell proliferation assay with 10 ~ (10) ~ 20 ~ (30) 渭 mol/L KY3 and flow cytometry, and the apoptosis and cell cycle distribution of EC109 cells were detected by flow cytometry, and the proliferation of EC109 cells was determined by flow cytometry, and the apoptosis and cell cycle distribution of EC109 cells were determined by flow cytometry. The effect of KY3 on the structure of microtubules in EC109 cells was observed by immunofluorescence method. The morphology of EC109 nucleus after KY3 was observed by Hoechst 33258 staining. Results KY3 could inhibit the proliferation of EC109 cells in vitro. After treatment with KY3, the cell cycle of EC109 cells was blocked in G _ 2 / M phase and apoptosis was induced by P0.05KY3. The microtubule was destroyed after treatment with KY3. With the increase of KY3 dose, 伪 -tubulin degradation and typical apoptotic morphology such as nuclear pyknosis and concentrated staining were observed. Conclusion: KY3 can inhibit the proliferation of EC109 cells in vitro, and the mechanism may be related to the cell cycle arrest and apoptosis induced by destroying the microtubule structure of G _ 2 / M phase.
【作者单位】：郑州大学医院检验科;郑州大学药学院;郑州大学基础医学院人体解剖学系;
【基金】：国家自然科学基金面上项目81430085,21372206,81172937 河南省科技厅基础与前沿项目142300410038 河南省教育厅基金资助项目17A310006 第59批国家博士后基金面上资助项目2016M592314
【分类号】：R735.1

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